Recombinant Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)
![Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-315391-ab251238408132antitrkatrkbtrkcphosphoy496y516y516antibodyepr19140bsaandazidefreewesternblot.jpg "Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19140] to TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516)
- Suitable for: Dot blot, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140]
See all TrkA+TrkB+TrkC primary antibodies -
Description
Rabbit monoclonal [EPR19140] to TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag; HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes; PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19140 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
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| WB |
1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 92 kDa).
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| Notes |
|---|
|
Dot blot
1/1000. |
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WB
1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 92 kDa). |
Target
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Cellular localization
TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane. -
Database links
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 4916 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 18213 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 29613 Rat
see all
Images
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Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution
Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-261152-anti-trka-phospho-y490-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-western-blot.jpg)
Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/10000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y516A mutant) with Myc-tag
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).
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![Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-261151-anti-trka-phospho-y490-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-western-blot.jpg)
Western blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).
-
![Dot Blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-261149-anti-trka-phospho-y490-trkb-phospho-y516-trkc-phospho-y516-antibody-epr19140-dot-blot.jpg)
Dot Blot - Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)Dot blot analysis of TrkA (phospho Y496), TrkB (phospho Y516), and TrkC (phospho Y516) peptides labeled with ab197071 at 1/1000 dilution.
Lane 1: TrkB (phospho Y516) phospho peptide;
Lane 2: TrkB Non-phospho peptide;
Lane 3: TrkA (phospho Y496) phospho peptide;
Lane 4: TrkA Non-phospho peptide;
Lane 5: TrkC (phospho Y516) phospho peptide;
Lane6: TrkC Non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Note: ab197071 reacts with TrkB (phospho Y516)/ TrkA (phospho Y496)/ TrkC (phospho Y516) according to the dot blot data.
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![Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)](https://www.abcam.com/ps/products/197/ab197071/Images/ab197071-5-benefits-of-recombinant-antibodies.png)
Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab197071 has been referenced in 1 publication.
- Teramura Y et al. Identification of Novel Fusion Genes in Bone and Soft Tissue Sarcoma and Their Implication in the Generation of a Mouse Model. Cancers (Basel) 12:N/A (2020). PubMed: 32825119