Overview

  • Product name

  • Description

    Rabbit polyclonal to TrkB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    The entire extracellular domain (corresponding to residues 1 to 429) of rat TrkB.

  • Positive control

    • Mouse brain membrane protein preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab33655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19923466
IHC-P Use at an assay dependent concentration. PubMed: 22646479
IP Use at an assay dependent concentration. PubMed: 23729812
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).

Target

  • Function

    Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • Tissue specificity

    Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ligand-mediated auto-phosphorylation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic receptor tyrosine kinase 2 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

Images

  • Anti-TrkB antibody (ab33655) at 1 µg/ml + Mouse brain membrane protein

    Predicted band size: 92 kDa

  • Immunohistochemical analysis of paraffin embedded mouse P29-RA lung section (10µm thick) labelling TrkB with ab33655 at 1:200 dilution. The secondary antibody was a peroxidase-labeled, polymer-conjugated goat anti-rabbit secondary antibody. Sections were stained with hematoxylin and eosin (H&E). Red arrow indicates bronchiolar epithelial cells, green arrow indicates type II pneumocyte and blue arrow indicates endothelial cells.

References

This product has been referenced in:

  • Bao Y  et al. Methyl 3,4-dihydroxybenzoate protects retina in a mouse model of acute ocular hypertension through multiple pathways. Exp Eye Res 181:15-24 (2019). Read more (PubMed: 30633922) »
  • Nie S  et al. 7,8-Dihydroxyflavone Protects Nigrostriatal Dopaminergic Neurons from Rotenone-Induced Neurotoxicity in Rodents. Parkinsons Dis 2019:9193534 (2019). Read more (PubMed: 30944722) »
See all 17 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain - Cerebellar granule cells - DIV3)
Loading amount
30 µg
Specification
Brain - Cerebellar granule cells - DIV3
Treatment
100 ng/ml human BDNF for 6 minutes
Gel Running Conditions
Reduced Denaturing (8% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 12 2013

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Brain - Cerebellar granule cells - DIV3)
Total protein in input
700 µg
Specification
Brain - Cerebellar granule cells - DIV3
Treatment
100 ng/ml human BDNF for 6 minutes
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

Submitted Mar 12 2013

Answer

Thank you for contacting us. Having compared the anti-phospho antibodies provided by Santa Cruz to those of Abcam (ab52191 and ab51187) they appear to be suitable for the same application in targeting the phosphorylated tyrosine-705 and -515 of TrkB. There are three isoforms of human TrkB referenced by SwissProt (Q16620) of 92, 53 and 59 kDa. However, TrkB exists as variably glycosylated entities with the major forms having molecular weights of ~145 kDa, and the unglycosylated form of ~95kD kDa. The product in the western blot identified as a degradation product is therefore more likely the unglycosylated form of the protein. Thank you for bringing this to our attention we will be updating our datasheet accordingly. ab52191, ab51187 and ab51190 all target the C-terminal domain while ab33655 is directed against the extracellular domain. All of the antibodies should be able to identify both the glycosylated and unglycosylated forms of the protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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