Product nameAnti-TrkB antibody
See all TrkB primary antibodies
DescriptionRabbit polyclonal to TrkB
Tested applicationsSuitable for: ICC/IF, IHC-P, IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
The entire extracellular domain (corresponding to residues 1 to 429) of rat TrkB.
- Mouse brain membrane protein preparation.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19923466|
|IHC-P||Use at an assay dependent concentration. PubMed: 22646479|
|IP||Use at an assay dependent concentration. PubMed: 23729812|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).|
FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain.
- Information by UniProt
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
Anti-TrkB antibody (ab33655) at 1 µg/ml + Mouse brain membrane protein
Predicted band size: 92 kDa
Immunohistochemical analysis of paraffin embedded mouse P29-RA lung section (10µm thick) labelling TrkB with ab33655 at 1:200 dilution. The secondary antibody was a peroxidase-labeled, polymer-conjugated goat anti-rabbit secondary antibody. Sections were stained with hematoxylin and eosin (H&E). Red arrow indicates bronchiolar epithelial cells, green arrow indicates type II pneumocyte and blue arrow indicates endothelial cells.
This product has been referenced in:
- Bao Y et al. Methyl 3,4-dihydroxybenzoate protects retina in a mouse model of acute ocular hypertension through multiple pathways. Exp Eye Res 181:15-24 (2019). Read more (PubMed: 30633922) »
- Nie S et al. 7,8-Dihydroxyflavone Protects Nigrostriatal Dopaminergic Neurons from Rotenone-Induced Neurotoxicity in Rodents. Parkinsons Dis 2019:9193534 (2019). Read more (PubMed: 30944722) »