Overview

  • Product name

    Anti-TrkB antibody [EPR1294]
    See all TrkB primary antibodies
  • Description

    Rabbit monoclonal [EPR1294] to TrkB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TrkB aa 100-200 (internal sequence). The exact sequence is proprietary.
    (Peptide available as ab195463)

  • Positive control

    • Fetal brain, Human hippocampus and Human cerebellum lysates; Human brain tissue
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: Internal testing data in Western Blot indicate that this antibody is not suitable for these species.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with TrkB peptide (ab195463).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
    • Tissue specificity

      Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Ligand-mediated auto-phosphorylation.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI848316 antibody
      • BDNF tropomyosine receptor kinase B antibody
      • BDNF/NT 3 growth factors receptor antibody
      • BDNF/NT-3 growth factors receptor antibody
      • Brain derived neurotrophic factor receptor antibody
      • C030027L06Rik antibody
      • EC 2.7.10.1 antibody
      • GP145 TrkB antibody
      • GP145-TrkB antibody
      • GP145-TrkB/GP95-TrkB antibody
      • GP95 TrkB antibody
      • Neurotrophic receptor tyrosine kinase 2 antibody
      • Neurotrophic tyrosine kinase receptor type 2 antibody
      • Neurotrophin receptor tyrosine kinase type 2 antibody
      • NTRK 2 antibody
      • Ntrk2 antibody
      • NTRK2_HUMAN antibody
      • Obesity, hyperphagia, and developmental delay, included antibody
      • RATTRKB1 antibody
      • Tkrb antibody
      • Trk B antibody
      • Trk-B antibody
      • TRKB antibody
      • TrkB tyrosine kinase antibody
      • TRKB1 antibody
      • Tropomyosin related kinase B antibody
      • tyrosine kinase receptor B antibody
      • Tyrosine receptor kinase B antibody
      see all

    Images

    • All lanes : Anti-TrkB antibody [EPR1294] (ab134155) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human hippocampus lysate
      Lane 3 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 92 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab134155 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab134155 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab134155 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134155 showing -ve staining.

    References

    ab134155 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1 buffer
    Permeabilization
    No
    Specification
    cerebellum
    Fixative
    formalin

    Mr. Emmanuel Bouchaert

    Verified customer

    Submitted Oct 06 2016

    Answer

    The rabbit monoclonal antibodies ab134155 and ab81288 will both have strong interaction with Protein G coated beads and would be suitable for IP using these.

    Read More

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