Product nameAnti-TrkB antibody [EPR1294]
See all TrkB primary antibodies
DescriptionRabbit monoclonal [EPR1294] to TrkB
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Human
Synthetic peptide within Human TrkB aa 100-200 (internal sequence). The exact sequence is proprietary.
(Peptide available as
- Fetal brain, Human hippocampus and Human cerebellum lysates; Human brain tissue
A trial size is available to purchase for this antibody.
Mouse, Rat: Internal testing data in Western Blot indicate that this antibody is not suitable for these species.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab134155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with TrkB peptide (ab195463).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain.
- Information by UniProt
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
All lanes : Anti-TrkB antibody [EPR1294] (ab134155) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human hippocampus lysate
Lane 3 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab134155 at 1/100 dilution.
Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab134155 showing -ve staining.
Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab134155 showing -ve staining.
Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134155 showing -ve staining.
ab134155 has not yet been referenced specifically in any publications.