Overview

  • Product name

    Anti-TrkB antibody [EPR17805-146]
    See all TrkB primary antibodies
  • Description

    Rabbit monoclonal [EPR17805-146] to TrkB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. within Mouse TrkB aa 100-450. The exact sequence is proprietary.
    Database link: P15209

  • Positive control

    • WB: Mouse TrkB active protein; mouse and rat brain lysates. IHC-P: Mouse and rat cerebral cortex tissues. IP: Mouse brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 140, 90 kDa (predicted molecular weight: 92 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/50.

Target

  • Function

    Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • Tissue specificity

    Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ligand-mediated auto-phosphorylation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic receptor tyrosine kinase 2 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

Images

  • Anti-TrkB antibody [EPR17805-146] (ab187041) at 1/5000 dilution + Mouse TrkB active protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 75-85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse TrkB active protein contains aa1-429 with a C-terminal poly histidine-tag.

  • All lanes : Anti-TrkB antibody [EPR17805-146] (ab187041) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140,90 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 140 kDa observed MW which is higher than the predicted one is due to the glycosylation modification.

    90 kDa corresponds to the truncated form, as observed in the literature (PMID:24860020).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and membrane staining on mouse cerebral cortex is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic and membrane staining on rat cerebral cortex is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse liver [PMID: 2555172].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • TrkB was immunoprecipitated from 1 mg of mouse brain lysate with ab187041 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab187041 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain lysate 10µg (Input).

    Lane 2: ab187041 IP in mouse brain lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab187041 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

This product has been referenced in:

  • Olenick MA  et al. Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons. J Cell Biol 218:220-233 (2019). Read more (PubMed: 30373907) »
  • Zhang Y  et al. nNOS-CAPON interaction mediates amyloid-ß-induced neurotoxicity, especially in the early stages. Aging Cell 17:e12754 (2018). Read more (PubMed: 29577585) »
See all 3 Publications for this product

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