Overview

  • Product name

    Anti-TrkB (phospho S479) antibody
    See all TrkB primary antibodies
  • Description

    Rabbit polyclonal to TrkB (phospho S479)
  • Host species

    Rabbit
  • Specificity

    In rodent TrkB, the immunogen corresponds to aa 471-483 (pS478).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human TrkB aa 472-485 (phospho S479).
    Sequence:

    SNDDDSA[pS]PLHHIS


    Database link: Q16620

  • Positive control

    • WB: NSC34 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 92 kDa.

Target

  • Function

    Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • Tissue specificity

    Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ligand-mediated auto-phosphorylation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic receptor tyrosine kinase 2 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

Images

  • All lanes : Anti-TrkB (phospho S479) antibody (ab228507) at 1 µg

    Lane 1 : NSC34 treated with retinoic acid, cell lysate
    Lane 2 : NSC34 treated with retinoic acid and BDNF, cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit HRP at 1/6000 dilution

    Developed using the ECL technique.

    Predicted band size: 92 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% skimmed milk in TBST.

    Primary antibody incubation: overnight at 4°C.

References

This product has been referenced in:

  • Luo H  et al. Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway. Front Pharmacol 10:395 (2019). Read more (PubMed: 31040784) »
See 1 Publication for this product

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