Product nameAnti-TrkB (phospho S479) antibody
See all TrkB primary antibodies
DescriptionRabbit polyclonal to TrkB (phospho S479)
SpecificityIn rodent TrkB, the immunogen corresponds to aa 471-483 (pS478).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Chicken, Human
- WB: NSC34 cell lysate.
FormLyophilised:Reconstitute in 50 µL sterile distilled water. Centrifuge to remove any insoluble material. After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 3% Trehalose
Concentration information loading...
Our Abpromise guarantee covers the use of ab228507 in the following tested applications.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 92 kDa.|
FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain.
- Information by UniProt
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
All lanes : Anti-TrkB (phospho S479) antibody (ab228507) at 1 µg
Lane 1 : NSC34 treated with retinoic acid, cell lysate
Lane 2 : NSC34 treated with retinoic acid and BDNF, cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit HRP at 1/6000 dilution
Developed using the ECL technique.
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% skimmed milk in TBST.
Primary antibody incubation: overnight at 4°C.
This product has been referenced in:
- Luo H et al. Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway. Front Pharmacol 10:395 (2019). Read more (PubMed: 31040784) »