Recombinant
RabMAb

Recombinant Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908)

Overview

  • Product name

    Anti-TrkB (phospho Y705) antibody [EPR22298-67]
    See all TrkB primary antibodies
  • Description

    Rabbit monoclonal [EPR22298-67] to TrkB (phospho Y705)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: CHIPseq,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse TrkB aa 650-750. The exact sequence is proprietary.
    Database link: P15209

  • Positive control

    • WB: HEK-293T transfected with mouse TrkB; HEK-293T transfected with human TrkB; PC-12 cells treated with 100ng/ml NGF.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab229908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.

 

 

WB 1/1000. Predicted molecular weight: 92 kDa.
  • Application notes
    Is unsuitable for CHIPseq,ICC/IF or IHC-P.
  • Target

    • Function

      Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
    • Tissue specificity

      Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Ligand-mediated auto-phosphorylation.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI848316 antibody
      • BDNF tropomyosine receptor kinase B antibody
      • BDNF/NT 3 growth factors receptor antibody
      • BDNF/NT-3 growth factors receptor antibody
      • Brain derived neurotrophic factor receptor antibody
      • C030027L06Rik antibody
      • EC 2.7.10.1 antibody
      • GP145 TrkB antibody
      • GP145-TrkB antibody
      • GP145-TrkB/GP95-TrkB antibody
      • GP95 TrkB antibody
      • Neurotrophic receptor tyrosine kinase 2 antibody
      • Neurotrophic tyrosine kinase receptor type 2 antibody
      • Neurotrophin receptor tyrosine kinase type 2 antibody
      • NTRK 2 antibody
      • Ntrk2 antibody
      • NTRK2_HUMAN antibody
      • Obesity, hyperphagia, and developmental delay, included antibody
      • RATTRKB1 antibody
      • Tkrb antibody
      • Trk B antibody
      • Trk-B antibody
      • TRKB antibody
      • TrkB tyrosine kinase antibody
      • TRKB1 antibody
      • Tropomyosin related kinase B antibody
      • tyrosine kinase receptor B antibody
      • Tyrosine receptor kinase B antibody
      see all

    Images

    • All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/1000 dilution

      Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
      Lane 2 : PC-12 treated with 100ng/ml NGF (ab9796) for 5 minutes at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 92 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The phosphorylation sites are conserved between TrkA and TrkB: Tyr674/675 of TrkA corresponds to Tyr706/707 in TrkB. Therefore, the TrkB (phospho T705) antibody can detect the phosphorylation of TrkA T674/675 in PC-12 cells treated with NGF. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23951412).

        Exposure time: 3 minutes

    • All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/5000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with human TrkB (WT) expression vector containing a myc-His-tag®, whole cell lysate (Untreated membrane)
      Lane 3 : HEK-293T transfected with human TrkB (WT) expression vector containing a myc-His-tag®, whole cell lysate (Phosphatase treated membrane)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 92 kDa
      Observed band size: None kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24959744). Besides, TrkB auto-phosphorylation was observed when TrkB was over-expressed in HEK293 cells, which has been reported in the literature (PMID: 25632160). 

      Exposure time: 7 seconds

    • All lanes : Anti-TrkB (phospho Y705) antibody [EPR22298-67] (ab229908) at 1/5000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, whole cell lysate
      Lane 3 : HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, then treated with 50ng/ml BDNF (ab9794) for 10 minutes, whole cell lysate (Untreated membrane)
      Lane 4 : HEK-293T transfected with mouse TrkB (WT) expression vector containing a myc-His-tag®, starved for 6 hours, then treated with 50ng/ml BDNF (ab9794) for 10 minutes, whole cell lysate (Phosphatase treated membrane)
      Lane 5 : HEK-293T transfected with mouse TrkB (mutant Y705A) expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 92 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24959744). Besides, TrkB auto-phosphorylation was observed when TrkB was over-expressed in HEK293 cells, which has been reported in the literature (PMID: 25632160). 

      Exposure time: 48 seconds

    • Dot blot analysis using ab229908 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

      Lane 1: TrkB (phospho T705) peptide (aa697-708)

      Lane 2: TrkB (phospho T705) peptide (aa702-712)

      Lane 3: TrkB non-phospho peptide (aa697-712)

      Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    References

    ab229908 has not yet been referenced specifically in any publications.

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