Product nameAnti-TrkB (phospho Y706) antibody [EPR19399]
See all TrkB primary antibodies
DescriptionRabbit monoclonal [EPR19399] to TrkB (phospho Y706)
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TrkB aa 700-800 (phospho Y706). The exact sequence is proprietary.
Database link: Q16620
- WB: HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag; HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab197072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 92 kDa).|
FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain.
- Information by UniProt
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
All lanes : Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 second
Blocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).
Dot blot analysis of TrkB (phospho Y706) labeled with ab197072 at 1/1000 dilution.
Lane 1: TrkB (phospho Y706) phospho peptide;
Lane 2: TrkB Non-phospho peptide;
Lane 3: TrkA (phospho Y680) phospho peptide;
Lane 4: TrkA Non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Note: ab197072 has weak cross reactivity with TrkA (phospho Y680) according to the dot blot data.
ab197072 has not yet been referenced specifically in any publications.