Recombinant Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)
![Western blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)](https://www.abcam.com/ps/products/197/ab197072/Images/ab197072-261154-anti-trkb-phospho-y706-antibody-epr19399-western-blot.jpg "Western blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19399] to TrkB (phospho Y706)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-TrkB (phospho Y706) antibody [EPR19399]
See all TrkB primary antibodies -
Description
Rabbit monoclonal [EPR19399] to TrkB (phospho Y706) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag; HEK-293 whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19399 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB | (1) |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 92 kDa).
|
| Notes |
|---|
|
Dot blot
1/1000. |
|
WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 92 kDa). |
Target
-
Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4915 Human
- Omim: 600456 Human
- SwissProt: Q16620 Human
- Unigene: 494312 Human
- Unigene: 712776 Human
-
Alternative names
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
Images
-
Western blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)All lanes : Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y706A mutant) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers. (PMID: 23115189 and 20064930).
-
![Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)](https://www.abcam.com/ps/products/197/ab197072/Images/ab197072-261153-anti-trkb-phospho-y706-antibody-epr19399-dot-blot.jpg)
Dot Blot - Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)Dot blot analysis of TrkB (phospho Y706) labeled with ab197072 at 1/1000 dilution.
Lane 1: TrkB (phospho Y706) phospho peptide;
Lane 2: TrkB Non-phospho peptide;
Lane 3: TrkA (phospho Y680) phospho peptide;
Lane 4: TrkA Non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Note: ab197072 has weak cross reactivity with TrkA (phospho Y680) according to the dot blot data.
-
![Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)](https://www.abcam.com/ps/products/197/ab197072/Images/ab197072-3-benefits-of-recombinant-antibodies.png)
Anti-TrkB (phospho Y706) antibody [EPR19399] (ab197072)
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab197072 has been referenced in 3 publications.
- Li K et al. Seahorse treatment improves depression-like behavior in mice exposed to CUMS through reducing inflammation/oxidants and restoring neurotransmitter and neurotrophin function. J Ethnopharmacol 250:112487 (2020). PubMed: 31857128
- Qin Z et al. Activation of tyrosine phosphatase PTP1B in pyramidal neurons impairs endocannabinoid signaling by tyrosine receptor kinase trkB and causes schizophrenia-like behaviors in mice. Neuropsychopharmacology 45:1884-1895 (2020). PubMed: 32610340
- Kawamoto M et al. The Novel Selective Pan-TRK Inhibitor ONO-7579 Exhibits Antitumor Efficacy Against Human Gallbladder Cancer In Vitro. Anticancer Res 38:1979-1986 (2018). PubMed: 29599313