Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain (hippocampus))
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE gel)
Loading amount
30 µg
Specification
Brain (hippocampus)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: +4°C · Diluent: 40% Glycerol, 0.01%NaN3, 5% BSA in PBS
Dilution
1/330
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate, BioRad
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemi Hi Sensitivity
Negative control
None
Exposure
20 second(s)
Bands
Specific: none kDa Non-specific: 60, 70 kDa
Positive control
pTrkB expressed in brain tissue, our samples were as positive controls
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Aug 04 2020