Overview

  • Product name

    Anti-TRM1 antibody - C-terminal
    See all TRM1 primary antibodies
  • Description

    Rabbit polyclonal to TRM1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human TRM1 aa 609-659 (C terminal). The exact sequence is proprietary. NP_060192.1.
    Sequence:

    FKEGTCQRGDQC CYSHSPPTPR VSADAAPDCP ETSNQTPPGP GAAAGPGID


    Database link: Q9NXH9

  • Positive control

    • 293T, HeLa, and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab185997 was affinity purified using an epitope specific to TRM1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 72 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.
  • Sequence similarities

    Contains 1 C3H1-type zinc finger.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2)G26)dimethyltransferase antibody
    • 2-dimethylguanosine-26 methyltransferase antibody
    • FLJ20244 antibody
    • N(2) N(2) dimethylguanosine tRNA methyltransferase antibody
    • N(2)-N(2)) methyltransferase antibody
    • TRM 1 antibody
    • TRM1 tRNA methyltransferase 1 homolog antibody
    • TRM1_HUMAN antibody
    • TRMT 1 antibody
    • Trmt1 antibody
    • tRNA (guanine(26)-N(2))-dimethyltransferase antibody
    • tRNA 2 2 dimethylguanosine 26 methyltransferase antibody
    • tRNA 2 antibody
    • tRNA(guanine 26 N(2) N(2)) methyltransferase antibody
    • tRNA(guanine-26 antibody
    • tRNA(m(2 2)G26)dimethyltransferase antibody
    • tRNA(m(2 antibody
    see all

Images

  • Detection of TRM1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185997 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab185997 was used at 1µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.

  • All lanes : Anti-TRM1 antibody - C-terminal (ab185997) at 1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 72 kDa


    Exposure time: 3 minutes

References

ab185997 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185997.
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