Key features and details
- Rabbit polyclonal to TRM1 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRM1 antibody - C-terminal
See all TRM1 primary antibodies
DescriptionRabbit polyclonal to TRM1 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Chimpanzee, Gorilla, Orangutan
- 293T, HeLa, and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab185997 was affinity purified using an epitope specific to TRM1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab185997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 72 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionDimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.
Sequence similaritiesContains 1 C3H1-type zinc finger.
- Information by UniProt
- 2)G26)dimethyltransferase antibody
- 2-dimethylguanosine-26 methyltransferase antibody
- FLJ20244 antibody
Detection of TRM1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185997 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab185997 was used at 1µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-TRM1 antibody - C-terminal (ab185997) at 1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 3 minutes
ab185997 has not yet been referenced specifically in any publications.