Anti-TRMT2B antibody - N-terminal (ab170278)

Product name

Anti-TRMT2B antibody - N-terminal
Description

Rabbit polyclonal to TRMT2B - N-terminal
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human TRMT2B aa 143-172 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q96GJ1
Positive control
- K562 cell lysate; Human skeletal muscle tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab170278 is purified through a protein A column, followed by immunogen affinity purification.
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab170278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB		1/100 - 1/500. Predicted molecular weight: 56 kDa.

Function

Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation.
Sequence similarities

Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family.
Target information above from: UniProt accession Q96GJ1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 79979 Human
- SwissProt: Q96GJ1 Human
- Unigene: 496501 Human
Alternative names
- Chromosome X open reading frame 34 antibody
- CXorf34 antibody
- dJ341D10.3 antibody
- FLJ12687 antibody
- Putative methyltransferase CXorf34 antibody
- TRM2 homolog antibody
- TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) antibody
- TRM2 tRNA methyltransferase 2 homolog B antibody
- TRM2_HUMAN antibody
- TRMT2B antibody
- tRNA (uracil 5 )methyltransferase homolog antibody
- tRNA (uracil(54)-C(5))-methyltransferase homolog antibody
- tRNA (uracil-5-)-methyltransferase homolog antibody
- tRNA methyltransferase 2 homolog B antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRMT2B antibody - N-terminal (ab170278)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling TRMT2B with ab170278 at 1/50 dilution.
Western blot - Anti-TRMT2B antibody - N-terminal (ab170278)

Anti-TRMT2B antibody - N-terminal (ab170278) at 1/100 dilution + K562 cell lysate at 35 µg

Predicted band size: 56 kDa

ab170278 has not yet been referenced specifically in any publications.

Publishing research using ab170278? Please let us know so that we can cite the reference in this datasheet.

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