Anti-TRMT2B antibody - N-terminal (ab170278)

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Overview

  • Product name
    Anti-TRMT2B antibody - N-terminal
  • Description
    Rabbit polyclonal to TRMT2B - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRMT2B aa 143-172 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96GJ1

  • Positive control
    • K562 cell lysate; Human skeletal muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170278 is purified through a protein A column, followed by immunogen affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 56 kDa.

Target

  • Function
    Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family.
  • Information by UniProt
  • Database links
    • Alternative names
      • Chromosome X open reading frame 34 antibody
      • CXorf34 antibody
      • dJ341D10.3 antibody
      • FLJ12687 antibody
      • Putative methyltransferase CXorf34 antibody
      • TRM2 homolog antibody
      • TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) antibody
      • TRM2 tRNA methyltransferase 2 homolog B antibody
      • TRM2_HUMAN antibody
      • TRMT2B antibody
      • tRNA (uracil 5 )methyltransferase homolog antibody
      • tRNA (uracil(54)-C(5))-methyltransferase homolog antibody
      • tRNA (uracil-5-)-methyltransferase homolog antibody
      • tRNA methyltransferase 2 homolog B antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRMT2B antibody - N-terminal (ab170278)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRMT2B antibody - N-terminal (ab170278)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling TRMT2B with ab170278 at 1/50 dilution.

    • Western blot - Anti-TRMT2B antibody - N-terminal (ab170278)
      Western blot - Anti-TRMT2B antibody - N-terminal (ab170278)
      Anti-TRMT2B antibody - N-terminal (ab170278) at 1/100 dilution + K562 cell lysate at 35 µg

      Predicted band size: 56 kDa

    References

    ab170278 has not yet been referenced specifically in any publications.

    Publishing research using ab170278? Please let us know so that we can cite the reference in this datasheet.

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