Anti-TROP2 antibody [162-46.2] (ab79976)
![Flow Cytometry - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.com/ps/products/79/ab79976/Images/ab79976-296165-anti-trop2-antibody-162-462-flow-cytometry.jpg "Flow Cytometry - Anti-TROP2 antibody [162-46.2] (ab79976)")
Key features and details
- Mouse monoclonal [162-46.2] to TROP2
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TROP2 antibody [162-46.2]
See all TROP2 primary antibodies -
Description
Mouse monoclonal [162-46.2] to TROP2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P Human
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Immunogen
Tissue, cells or virus corresponding to Human TROP2. Human choriocarcinoma cell line BeWo.
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Positive control
- IHC-P: Human placenta tissue. ICC/IF: JEG3 and MCF7 cells. Flow Cyt: JEG3 and MCF7 cells.
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General notes
This antibody clone is manufactured by Abcam.
Isotype: IgG1 (Igh-4a allotype)
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
162-46.2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| Flow Cyt |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May function as a growth factor receptor. -
Tissue specificity
Placenta, pancreatic carcinoma cell lines. -
Involvement in disease
Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. -
Sequence similarities
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4070 Human
- Omim: 137290 Human
- SwissProt: P09758 Human
- Unigene: 23582 Human
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Alternative names
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
Images
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Flow Cytometry - Anti-TROP2 antibody [162-46.2] (ab79976)
Overlay histogram showing JEG3 cells stained with ab79976 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab79976, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (monoclonal) (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody gave a positive signal in JEG3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.com/ps/products/79/ab79976/Images/ab79976-95365-anti-trop2-antibody-162-462-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [162-46.2] (ab79976)MCF7 cells stained for TROP2 (colored green) using ab79976 in ICC/IF. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.com/ps/products/79/ab79976/Images/ab79976-95364-anti-trop2-antibody-162-462-flow-cytometry.jpg)
Flow Cytometry - Anti-TROP2 antibody [162-46.2] (ab79976)Overlay histogram showing MCF7 cells stained with ab79976 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MCF7 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.com/ps/products/79/ab79976/Images/ab79976-95366-anti-trop2-antibody-162-462-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [162-46.2] (ab79976)ab79976 staining TROP2 in human 10 week placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Anti-mouse peroxidase IgG used as secondary antibody. Note staining of cytotrophoblast (CT) and syncytiotrophoblast (ST).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab79976 has been referenced in 4 publications.
- Grünherz L et al. Osteoidosis leads to altered differentiation and function of osteoclasts. J Cell Mol Med 24:5665-5674 (2020). PubMed: 32283567
- Im H et al. Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone. Proc Natl Acad Sci U S A 112:5613-8 (2015). PubMed: 25870273
- Viswanatha R et al. Interactome analysis reveals ezrin can adopt multiple conformational states. J Biol Chem 288:35437-51 (2013). ICC/IF ; Human . PubMed: 24151071
- Lipinski M et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc Natl Acad Sci U S A 78:5147-50 (1981). PubMed: 7029529