Anti-TROP2 antibody (ab64320)

Reviews (3)Q&A (1)
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody (ab64320)

    Key features and details

    • Rabbit polyclonal to TROP2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-TROP2 antibody [EPR20043] (ab214488)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-TROP2 antibody
      See all TROP2 primary antibodies

    • Description

      Rabbit polyclonal to TROP2

    • Host species

      Rabbit

    • Specificity

      Reacts specifically with human 35 kDa TROP2 protein.

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Pig, a wide range of other species

    • Immunogen

      Synthetic peptide corresponding to Human TROP2 (C terminal).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • Storage buffer

      Constituent: Whole serum
    • Concentration information loading...

    • Purity

      Whole antiserum

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64320 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF (1)
    Use a concentration of 5 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 5 µg/ml.

    Target

    • Function

      May function as a growth factor receptor.

    • Tissue specificity

      Placenta, pancreatic carcinoma cell lines.

    • Involvement in disease

      Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.

    • Sequence similarities

      Belongs to the EPCAM family.
      Contains 1 thyroglobulin type-1 domain.

    • Post-translational
      modifications

      The N-terminus is blocked.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cell surface glycoprotein Trop 2 antibody
      • Cell surface glycoprotein Trop-2 antibody
      • Cell surface glycoprotein Trop2 antibody
      • Epithelial glycoprotein 1 antibody
      • GA733 1 antibody
      • GA7331 antibody
      • M1S 1 antibody
      • M1S1 antibody
      • Membrane component chromosome 1 surface marker 1 antibody
      • Pancreatic carcinoma marker protein GA733 1 antibody
      • Pancreatic carcinoma marker protein GA733-1 antibody
      • Pancreatic carcinoma marker protein GA7331 antibody
      • TACD 2 antibody
      • TACD2_HUMAN antibody
      • TACSTD 2 antibody
      • Tacstd2 antibody
      • Trop 2 antibody
      • Trop2 antibody
      • Tumor associated calcium signal transducer 2 precursor antibody
      • Tumor-associated calcium signal transducer 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody (ab64320)
      Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody (ab64320)

      ICC/IF image of ab64320 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References (0)

    Publishing research using ab64320? Please let us know so that we can cite the reference in this datasheet.

    ab64320 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-TROP2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Prostate)
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 08 2012

    Immunocytochemistry abreview for Anti-TROP2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (prostate)
    Permeabilization
    No
    Specification
    prostate
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Methanol
    Read More

    Dr. Olayinka Oloko

    Verified customer

    Submitted Dec 05 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TROP2 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (prostate)
    Permeabilization
    No
    Specification
    prostate
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Olayinka Oloko

    Verified customer

    Submitted Dec 05 2011

    Question

    schreibe grade Abreviews fuer ab49730 und ab79976 fuer den Test im Schwein, leider nicht geklappt.
    Gibt es Alternativen zu testen? Mit Testsample? Wurden andere AK schonmal auf Schwein getestet?

    Read More
    Answer

    Vielen Dank für Ihre Anrufe.

    Wie versprochen habe ich alle unsere Langerin- bzw. TACD2-Antikörper danach geprüft, ob sie schon einmal mit Schwein getestet wurden - was leider bei keinem der Fall ist.

    Daher habe ich die Immunogene auf Sequenzähnlichkeit mit dem Langerin bzw. TACD2 des Schweins (SwissProt IDs B3FVQ1 bzw. F1S7A4) untersucht, und die BLAST-Recherchen ergaben folgendes:

    Langerin

    ab68434 (Immunogen Aminosäuren 1-20, MTVEKEAPDAHFTVDKQNIS): 83%

    https://www.abcam.com/Langerin-antibody-ab68434.html (or use the following: https://www.abcam.com/Langerin-antibody-ab68434.html).

    ab125476: 79%

    https://www.abcam.com/Langerin-antibody-ab125476.html (or use the following: https://www.abcam.com/Langerin-antibody-ab125476.html).

    ab90959: 66%

    https://www.abcam.com/Langerin-antibody-L31-ab90959.html (or use the following: https://www.abcam.com/Langerin-antibody-L31-ab90959.html).

    ab22111: viele Treffer mit unterschiedlichen Ähnlichkeiten (ca. 70%), daher nicht empfehlenswert zu testen

    https://www.abcam.com/Langerin-antibody-ab22111.html (or use the following: https://www.abcam.com/Langerin-antibody-ab22111.html).

    TACD2

    ab65006 (Aminosäuren 130-180): 92%

    https://www.abcam.com/TROP2-antibody-ab65006.html (or use the following: https://www.abcam.com/TROP2-antibody-ab65006.html).

    ab58930 (Immunogen Aminosäuren 194-209, VHYEQPTIQIELRQNT): 86%

    https://www.abcam.com/TROP2-antibody-ab58930.html (or use the following: https://www.abcam.com/TROP2-antibody-ab58930.html).

    ab77551: 85%

    https://www.abcam.com/TROP2-antibody-ab77551.html (or use the following: https://www.abcam.com/TROP2-antibody-ab77551.html).

    ab89928: 85%

    https://www.abcam.com/TROP2-antibody-MM0588-49D6-ab89928.html (or use the following: https://www.abcam.com/TROP2-antibody-MM0588-49D6-ab89928.html).

    ab64320: Die Immunogensequenz muss im Labor nachgefragt werden.

    https://www.abcam.com/TROP2-antibody-ab64320.html (or use the following: https://www.abcam.com/TROP2-antibody-ab64320.html).

    Für diese Antikörper kann ich Ihnen wie zuvor wieder jeweils einen Testrabatt anbieten, d.h. wenn Sieeinen der Antikörper kaufen, uns Ihre Ergebnisse mit Schwein per Abreview mitteilen, bekommen Sie wieder jeweils einen kosten Primärantikörper zum Dank (und die Abpoints nicht zu vergessen) - https://www.abcam.com/collaborationdiscount.

    Dies wäre vor allem für den TACD2- Antikörper ab65006 zu empfehlen, den wir schon in der Immunhistochemiegetestet haben und daher wissen, dass er wenigstensin der Anwendung funktioniert - auch wenn die Spezies Schwein noch nichtvalidiert wurde. Allerdings ist mit einer Sequenzähnlichkeit von 92%die Wahrscheinlichkeit einer Kreuzreaktivität sehr hoch.Aus dem gleichen Grund würde sich auch ab64320 eignen; hier können wir aber gerne noch die Rückmeldung aus dem Labor abwarten. Alle anderen Antikörper wurden bisher noch nicht in der Immunhistochemie getestet, daher besteht hier ein doppeltes Risiko, da wir nicht wissen, ob die Antikörper für diese Anwendung geeignet sind.

    Sie können diese Antikörper auch wie besprochen kostenlos erhalten, wenn Sie sie mittels der Gutscheine von den vorherigen Testungen bestellen. Allerdings können wir in diesem Fall keine Garantie dafür übernehmen, wenn sienicht mit Schwein kreuzreagieren.

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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