Anti-TROP2 antibody (ab64320)

Reviews (3)Q&A (1)

Overview

  • Product name

    Anti-TROP2 antibody
    See all TROP2 primary antibodies

  • Description

    Rabbit polyclonal to TROP2

  • Host species

    Rabbit

  • Specificity

    Reacts specifically with human 35 kDa TROP2 protein.

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, ICCmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, a wide range of other species

  • Immunogen

    Synthetic peptide corresponding to Human TROP2 (C terminal).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Constituent: Whole serum

  • Purity

    Whole antiserum

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 37 kDa.
ICC Use at an assay dependent concentration.

Target

  • Function

    May function as a growth factor receptor.

  • Tissue specificity

    Placenta, pancreatic carcinoma cell lines.

  • Involvement in disease

    Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.

  • Sequence similarities

    Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.

  • Post-translational
    modifications

    The N-terminus is blocked.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    • Epithelial glycoprotein 1 antibody
    • GA733 1 antibody
    • GA7331 antibody
    • M1S 1 antibody
    • M1S1 antibody
    • Membrane component chromosome 1 surface marker 1 antibody
    • Pancreatic carcinoma marker protein GA733 1 antibody
    • Pancreatic carcinoma marker protein GA733-1 antibody
    • Pancreatic carcinoma marker protein GA7331 antibody
    • TACD 2 antibody
    • TACD2_HUMAN antibody
    • TACSTD 2 antibody
    • Tacstd2 antibody
    • Trop 2 antibody
    • Trop2 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody (ab64320)
    Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody (ab64320)

    ICC/IF image of ab64320 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab64320 has not yet been referenced specifically in any publications.

Publishing research using ab64320? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-TROP2 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Prostate)
Permeabilization
No
Specification
Prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

Submitted Jun 08 2012

Question

schreibe grade Abreviews fuer ab49730 und ab79976 fuer den Test im Schwein, leider nicht geklappt.
Gibt es Alternativen zu testen? Mit Testsample? Wurden andere AK schonmal auf Schwein getestet?

Read More
Answer

Vielen Dank für Ihre Anrufe.

Wie versprochen habe ich alle unsere Langerin- bzw. TACD2-Antikörper danach geprüft, ob sie schon einmal mit Schwein getestet wurden - was leider bei keinem der Fall ist.

Daher habe ich die Immunogene auf Sequenzähnlichkeit mit dem Langerin bzw. TACD2 des Schweins (SwissProt IDs B3FVQ1 bzw. F1S7A4) untersucht, und die BLAST-Recherchen ergaben folgendes:

Langerin

ab68434 (Immunogen Aminosäuren 1-20, MTVEKEAPDAHFTVDKQNIS): 83%

https://www.abcam.com/index.html?datasheet=68434 (or use the following: https://www.abcam.com/index.html?datasheet=68434).

ab125476: 79%

https://www.abcam.com/index.html?datasheet=125476 (or use the following: https://www.abcam.com/index.html?datasheet=125476).

ab90959: 66%

https://www.abcam.com/index.html?datasheet=90959 (or use the following: https://www.abcam.com/index.html?datasheet=90959).

ab22111: viele Treffer mit unterschiedlichen Ähnlichkeiten (ca. 70%), daher nicht empfehlenswert zu testen

https://www.abcam.com/index.html?datasheet=22111 (or use the following: https://www.abcam.com/index.html?datasheet=22111).

TACD2

ab65006 (Aminosäuren 130-180): 92%

https://www.abcam.com/index.html?datasheet=65006 (or use the following: https://www.abcam.com/index.html?datasheet=65006).

ab58930 (Immunogen Aminosäuren 194-209, VHYEQPTIQIELRQNT): 86%

https://www.abcam.com/index.html?datasheet=58930 (or use the following: https://www.abcam.com/index.html?datasheet=58930).

ab77551: 85%

https://www.abcam.com/index.html?datasheet=77551 (or use the following: https://www.abcam.com/index.html?datasheet=77551).

ab89928: 85%

https://www.abcam.com/index.html?datasheet=89928 (or use the following: https://www.abcam.com/index.html?datasheet=89928).

ab64320: Die Immunogensequenz muss im Labor nachgefragt werden.

https://www.abcam.com/index.html?datasheet=64320 (or use the following: https://www.abcam.com/index.html?datasheet=64320).

Für diese Antikörper kann ich Ihnen wie zuvor wieder jeweils einen Testrabatt anbieten, d.h. wenn Sieeinen der Antikörper kaufen, uns Ihre Ergebnisse mit Schwein per Abreview mitteilen, bekommen Sie wieder jeweils einen kosten Primärantikörper zum Dank (und die Abpoints nicht zu vergessen) - https://www.abcam.com/collaborationdiscount.

Dies wäre vor allem für den TACD2- Antikörper ab65006 zu empfehlen, den wir schon in der Immunhistochemiegetestet haben und daher wissen, dass er wenigstensin der Anwendung funktioniert - auch wenn die Spezies Schwein noch nichtvalidiert wurde. Allerdings ist mit einer Sequenzähnlichkeit von 92%die Wahrscheinlichkeit einer Kreuzreaktivität sehr hoch.Aus dem gleichen Grund würde sich auch ab64320 eignen; hier können wir aber gerne noch die Rückmeldung aus dem Labor abwarten. Alle anderen Antikörper wurden bisher noch nicht in der Immunhistochemie getestet, daher besteht hier ein doppeltes Risiko, da wir nicht wissen, ob die Antikörper für diese Anwendung geeignet sind.

Sie können diese Antikörper auch wie besprochen kostenlos erhalten, wenn Sie sie mittels der Gutscheine von den vorherigen Testungen bestellen. Allerdings können wir in diesem Fall keine Garantie dafür übernehmen, wenn sienicht mit Schwein kreuzreagieren.

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Immunocytochemistry abreview for Anti-TROP2 antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (prostate)
Permeabilization
No
Specification
prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Methanol
Read More

Dr. Olayinka Oloko

Verified customer

Submitted Dec 05 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-TROP2 antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Permeabilization
No
Specification
prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Dr. Olayinka Oloko

Verified customer

Submitted Dec 05 2011

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