- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-TROP2 antibody
See all TROP2 primary antibodies -
DescriptionRabbit polyclonal to TROP2
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Host speciesRabbit
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SpecificityReacts specifically with human 35 kDa TROP2 protein.
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Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, WB, ICCmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig, a wide range of other species
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Immunogen
Synthetic peptide corresponding to Human TROP2 (C terminal).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: None
Constituents: Whole serum -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab64320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/200 - 1/2000. Predicted molecular weight: 37 kDa. | |
| ICC | Use at an assay dependent concentration. |
Target
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FunctionMay function as a growth factor receptor.
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Tissue specificityPlacenta, pancreatic carcinoma cell lines.
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Involvement in diseaseDefects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
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Sequence similaritiesBelongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 4070 Human
- Entrez Gene: 56753 Mouse
- Entrez Gene: 494343 Rat
- Omim: 137290 Human
- SwissProt: P09758 Human
- SwissProt: Q8BGV3 Mouse
- SwissProt: Q6P9Z6 Rat
- Unigene: 23582 Human
see all -
Alternative names
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody (ab64320)ICC/IF image of ab64320 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
ab64320 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"