Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)
Key features and details
- Mouse monoclonal [M005] to TROP2 - Extracellular domain
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-TROP2 antibody [M005] - Extracellular domain
See all TROP2 primary antibodies -
Description
Mouse monoclonal [M005] to TROP2 - Extracellular domain -
Host species
Mouse -
Specificity
The antibody detects denatured TROP2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot.
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Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human TROP2. Clone M005 was generated from a proprietary antigen related to the extracellular region of human TROP2 from the A431 epidermoid carcinoma cell line.
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Positive control
- WB: A431, MDA-MB-231 and MCF7 cell lysates. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M005 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 36 kDa.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 36 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
May function as a growth factor receptor. -
Tissue specificity
Placenta, pancreatic carcinoma cell lines. -
Involvement in disease
Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. -
Sequence similarities
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4070 Human
- Omim: 137290 Human
- SwissProt: P09758 Human
- Unigene: 23582 Human
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Alternative names
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
Images
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Paraformaldehyde-fixed A431 (Human epidermoid carcinoma cell line) cells labeling TROP2 using ab253293 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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All lanes : Anti-TROP2 antibody [M005] - Extracellular domain (ab253293) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate, native conditions
Lane 2 : A431 (Human epidermoid carcinoma cell line) cell lysate, denatured conditions
Lane 3 : MDA-MB-231 (Human cell line) cell lysate, native conditions
Lane 4 : MDA-MB-231 (Human cell line) cell lysate, denatured conditions
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) cell lysate, native conditions
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) cell lysate, denatured conditions
Predicted band size: 36 kDaLanes 1, 3 & 5: Native conditions.
Lanes 2, 4 & 6: Denaturing conditions.
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.
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Representative Standard Curve using ab253293 for ELISA capture of human recombinant TROP2 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab253293 has not yet been referenced specifically in any publications.