Recombinant
RabMAb

Recombinant Anti-TROP2 antibody [SP294] (ab227690)

Overview

  • Product name

    Anti-TROP2 antibody [SP294]
    See all TROP2 primary antibodies
  • Description

    Rabbit monoclonal [SP294] to TROP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human TROP2 aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: P09758

  • Positive control

    • WB: SK-BR-3 cell lysate. IHC-P: Human tonsil, breast, cervix, skin, lung, prostate, bladder, bladder transitional cell carcinoma, lung squamous cell carcinoma, skin squamous cell carcinoma, cervical squamous cell carcinoma, breast ductal cell carcinoma, pancreatic adenocarcinoma and prostate adenocarcinoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP294
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. 

WB 1/100. Predicted molecular weight: 36 kDa.

Target

  • Function

    May function as a growth factor receptor.
  • Tissue specificity

    Placenta, pancreatic carcinoma cell lines.
  • Involvement in disease

    Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
  • Sequence similarities

    Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    • Epithelial glycoprotein 1 antibody
    • GA733 1 antibody
    • GA7331 antibody
    • M1S 1 antibody
    • M1S1 antibody
    • Membrane component chromosome 1 surface marker 1 antibody
    • Pancreatic carcinoma marker protein GA733 1 antibody
    • Pancreatic carcinoma marker protein GA733-1 antibody
    • Pancreatic carcinoma marker protein GA7331 antibody
    • TACD 2 antibody
    • TACD2_HUMAN antibody
    • TACSTD 2 antibody
    • Tacstd2 antibody
    • Trop 2 antibody
    • Trop2 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling TROP2 with ab227690 at 1/100 dilution (2.47 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human bladder carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227690 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TROP2 with ab227690 at 1:100 dilution (2.47 ?g/ml). Heat mediated antigen retrieval with Bond� Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on epithelium of the human tonsil, performed on a Leica Biosystems BOND� RX instrument.
    The section was incubated with ab227690 for 30 mins at room temperature.
  • Anti-TROP2 antibody [SP294] (ab227690) at 1/100 dilution + SK-BR-3 (human mammary gland adenocarcinoma cell line) cell lysate

    Predicted band size: 36 kDa

  • Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervix tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast ductal cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for TROP2 using ab227690 at 1/100 dilution in immunohistochemical analysis.

References

ab227690 has not yet been referenced specifically in any publications.

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