Recombinant
RabMAb

Recombinant Anti-TROP2 antibody [SP295] (ab227691)

Overview

  • Product name

    Anti-TROP2 antibody [SP295]
    See all TROP2 primary antibodies
  • Description

    Rabbit monoclonal [SP295] to TROP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human TROP2 aa 100-200 (internal sequence). The exact sequence is proprietary.
    Database link: P09758

  • Positive control

    • WB: SK-BR-3 cell lysate. IHC-P: Human tonsil, skin, prostate, pancreas, breast, bladder, skin squamous cell carcinoma, prostate adenocarcinoma, lung squamous cell carcinoma, breast ductal cell carcinoma and bladder transitional cell carcinoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP295
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary incubation for 10 minutes at room temperature. 

WB 1/100. Predicted molecular weight: 36 kDa.

Primary incubation for 1 hour at room temperature.

Target

  • Function

    May function as a growth factor receptor.
  • Tissue specificity

    Placenta, pancreatic carcinoma cell lines.
  • Involvement in disease

    Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
  • Sequence similarities

    Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    • Epithelial glycoprotein 1 antibody
    • GA733 1 antibody
    • GA7331 antibody
    • M1S 1 antibody
    • M1S1 antibody
    • Membrane component chromosome 1 surface marker 1 antibody
    • Pancreatic carcinoma marker protein GA733 1 antibody
    • Pancreatic carcinoma marker protein GA733-1 antibody
    • Pancreatic carcinoma marker protein GA7331 antibody
    • TACD 2 antibody
    • TACD2_HUMAN antibody
    • TACSTD 2 antibody
    • Tacstd2 antibody
    • Trop 2 antibody
    • Trop2 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling TROP2 with ab227691 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human bladder carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228199 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling TROP2 with ab227691 at 1:100 dilution (1.01 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human skin, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228199 for 10 mins at room temperature.

  • Anti-TROP2 antibody [SP295] (ab227691) at 1/100 dilution + SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate

    Predicted band size: 36 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreas tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast ductal cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

References

ab227691 has not yet been referenced specifically in any publications.

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