Key features and details
- Rabbit polyclonal to Trophoblast specific protein alpha
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Trophoblast specific protein alpha antibody
DescriptionRabbit polyclonal to Trophoblast specific protein alpha
Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Trophoblast specific protein alpha aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse trophoblast tissue lysate as well as the following whole cell lysates: MEF1; NIH3T3; STO.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab104401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.|
RelevanceTrophoblast specific protein alpha is a marker of spongiotrophoblasts.
- Tb1 antibody
- Tpbp antibody
- Tpbpa antibody
All lanes : Anti-Trophoblast specific protein alpha antibody (ab104401) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Trophoblast Stem Cells (Mouse) Whole Cell Lysate
Lane 4 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 150 kDa, 255 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes
ICC/IF image of ab104401 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab104401, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MEF1 cells at 1µg/ml.
ab104401 has been referenced in 12 publications.
- Peng K et al. Derivation of Haploid Trophoblast Stem Cells via Conversion In Vitro. iScience 11:508-518 (2019). PubMed: 30635215
- Wattez JS et al. The platelet-derived growth factor receptor alpha promoter-directed expression of cre recombinase in mouse placenta. Dev Dyn 248:363-374 (2019). PubMed: 30843624
- Sharma A et al. miR-126 regulates glycogen trophoblast proliferation and DNA methylation in the murine placenta. Dev Biol 449:21-34 (2019). PubMed: 30771304
- Segura-Bayona S et al. Differential requirements for Tousled-like kinases 1 and 2 in mammalian development. Cell Death Differ 24:1872-1885 (2017). PubMed: 28708136
- Monsivais D et al. BMP7 Induces Uterine Receptivity and Blastocyst Attachment. Endocrinology 158:979-992 (2017). PubMed: 28324064