Product nameAnti-Trophoblast specific protein alpha antibody
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Trophoblast specific protein alpha aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse trophoblast tissue lysate as well as the following whole cell lysates: MEF1; NIH3T3; STO.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
|ELISA||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.|
RelevanceTrophoblast specific protein alpha is a marker of spongiotrophoblasts.
- Tb1 antibody
- Tpbp antibody
- Tpbpa antibody
All lanes : Anti-Trophoblast specific protein alpha antibody (ab104401) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Trophoblast Stem Cells (Mouse) Whole Cell Lysate
Lane 4 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 150 kDa, 255 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes
ICC/IF image of ab104401 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab104401, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MEF1 cells at 1µg/ml.
This product has been referenced in:
- Peng K et al. Derivation of Haploid Trophoblast Stem Cells via Conversion In Vitro. iScience 11:508-518 (2019). Read more (PubMed: 30635215) »
- Wattez JS et al. The platelet-derived growth factor receptor alpha promoter-directed expression of cre recombinase in mouse placenta. Dev Dyn 248:363-374 (2019). Read more (PubMed: 30843624) »