Product nameAnti-Tropoelastin antibody
DescriptionRabbit polyclonal to Tropoelastin
Interacts with tropoelastin and insoluble elastin (there are many shared epitopes between the two forms of the protein).
Tested applicationsSuitable for: ICC, IHC-Fr, IHC-P, ELISA, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide corresponding to Mouse Tropoelastin. ab21600 was generated to the N-terminal portion of mouse tropoelastin, the soluble precursor form of elastin.
- In Western Blot, this antibody gave a positive signal in human heart tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: Whole serum, 50% Glycerol
Our Abpromise guarantee covers the use of ab21600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/50 - 1/100.|
|IHC-Fr||1/50 - 1/100.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/1000 - 1/2000.|
|WB||1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).|
|IP||1/100 - 1/200.|
FunctionMajor structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Tissue specificityExpressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
Involvement in diseaseDefects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the elastin family.
modificationsElastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Cellular localizationSecreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
- Information by UniProt
- Elastin antibody
- ELN antibody
- ELN_HUMAN antibody
Anti-Tropoelastin antibody (ab21600) at 1/500 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This product has been referenced in:
- Fang L et al. Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells via interaction with tropoelastin mRNA. Int J Mol Med 43:1597-1610 (2019). Read more (PubMed: 30720050) »
- McCulley DJ et al. PBX transcription factors drive pulmonary vascular adaptation to birth. J Clin Invest 128:655-667 (2018). Read more (PubMed: 29251627) »