Overview

  • Product name
    Anti-Tropoelastin antibody
  • Description
    Rabbit polyclonal to Tropoelastin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, IHC-Fr, IHC-P, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide (containing sequences from exons 6-17)(Mouse).

  • Positive control
    • In Western Blot, this antibody gave a positive signal in human heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab21600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.
ELISA 1/1000 - 1/2000.
WB 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
IP 1/100 - 1/200.

Target

  • Function
    Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
  • Tissue specificity
    Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
  • Involvement in disease
    Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities
    Belongs to the elastin family.
  • Post-translational
    modifications
    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Elastin antibody
    • ELN antibody
    • ELN_HUMAN antibody
    • SVAS antibody
    • Tropoelastin antibody
    see all

Images

  • Anti-Tropoelastin antibody (ab21600) at 1/500 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

References

This product has been referenced in:
  • Gallardo-Rivera R  et al. Polyelectrolyte complex of Aloe vera, chitosan, and alginate produced fibroblast and lymphocyte viabilities and migration. Carbohydr Polym 192:84-94 (2018). Read more (PubMed: 29691038) »
  • Kato K  et al. Pulmonary pericytes regulate lung morphogenesis. Nat Commun 9:2448 (2018). Read more (PubMed: 29934496) »
See all 8 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - other (ECM protein extract)
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Loading amount
6 µg
Specification
ECM protein extract
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Christian Cassel

Verified customer

Submitted Sep 03 2018

Answer

Thank you for your enquiry.

I can confirm that ab21600 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your inquiry.

The lab said that they would think ab21607 & ab21610 would recognize the precursor of elastin, aka tropoelastin, but this hasn't been specifically tested.

I think I found an antibody that would work for you and that is ab21600, anti-tropoelastin. This works in mouse in ICC, IHC-Fr, IHC-P, ELISA, WB, andIP.

https://www.abcam.com/Tropoelastin-antibody-ab21600.html

I hope this information helps. Please contact us with any other questions.

Read More

Answer

Thank you for contacting us.

Neither ab21605 or ab21600 have been tested in plants, and we guarantee our products work in the tested species and applications stated on the datasheet.

ab21605, which has been discontinued, was generated against the full length protein (Human) and maybe had the ability to recognise the Tropoelastin in plant samples.

ab21600 is specifically directed against a synthetic peptide, not againstthe full length protein like ab21600. The immunogen sequence may not be present in the plant Tropoelastin, which would explain the results with this antibody.

I would suggest to test this antibody with the recommended positive control, human heart tissue lysate. To our knowledge, ab21600 has been tested only with Human samples and is not guaranteed to work in other species.

I hope this information is helpful to you and your customer.

Read More

Answer

Thank you for your inquiry.

Indeed, there is the risk that this antibody would not work on the human tropoelastin expressed in tobacco plant due to differential posttranslational modifications. However I think that the chances are high that the antibody is working and I would therefore exceptionnaly agree on covering this for the customer this time.

I hope you find this answer satisfactory. Please do not hesitate to contact us again, should you have any further questions.

Read More

Answer

Thank you for enquiry. I have been in touch with the source of this antibody and they have come back with a few comments. "Please can you tell me whether you intend to distinguish tropoelastin from insoluble elastin by IHC in in intact tissues. This will not work for several reasons. The most important is that under normal circumstances, tropoelastin is instantaneously crosslinked to insoluble elastin in tissues, so that there is never a pool of the soluble protein that can be detected separate from the insoluble fibers. Second, there are very few, if any, antibodies that see tropoelastin but not insoluble elastin. Likewise, most antibodies to insoluble elastin also see tropoelastin". According to the source of the antibody both ab21600 and ab21601 will react with insoluble elastin in tissues. I hope this information helps and that I have not misunderstood your question. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your phone call. The difference between these two antibodies are the immunogens that were used to produce the antibodies. Ab21600 was raised against a peptide containing sequences from exons 6-17 (mouse). Ab21601 was raised against a peptide containing sequences from exons 31-36 (mouse). Both antibodies have been tested for cross-reactivity with rat (as well as human, mouse and cow) and tested for application in Western blotting (as well as Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry). Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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