Key features and details
- Rabbit polyclonal to Tropomodulin 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Tropomodulin 1 antibody
See all Tropomodulin 1 primary antibodies
DescriptionRabbit polyclonal to Tropomodulin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant full length protein corresponding to Human Tropomodulin 1 aa 1-359.
MSYRRELEKYRDLDEDEILGALTEEELRTLENELDELDPDNALLPAGLRQ KDQTTKAPTGPFKREELLDHLEKQAKEFKDREDLVPYTGEKRGKVWVPKQ KPLDPVLESVTLEPELEEALANASDAELCDIAAILGMHTLMSNQQYYQAL SSSSIMNKEGLNSVIKPTQYKPVPDEEPNSTDVEETLERIKNNDPKLEEV NLNNIRNIPIPTLKAYAEALKENSYVKKFSIVGTRSNDPVAYALAEMLKE NKVLKTLNVESNFISGAGILRLVEALPYNTSLVEMKIDNQSQPLGNKVEM EIVSMLEKNATLLKFGYHFTQQGPRLRASNAMMNNNDLVRKRRLADLTGP IIPKCRSGV
Database link: P28289
- WB: Mouse brain and lung lysates; HeLa, K562, A549 and MCF7 whole cell lysates. IHC-P: Human heart and pancreatic tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- Mouse lung normal tissue lysate - total protein (ab29297)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- MCF7 whole cell lysate (ab3871)
- Mouse brain tissue lysate - total protein (ab4022)
- A549 whole cell lysate (ab7910)
- K562 whole cell lysate (ab7911)
Our Abpromise guarantee covers the use of ab230276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
FunctionBlocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton. May play an important role in regulating the organization of actin filaments by preferentially binding to a specific tropomyosin isoform at its N-terminus.
Tissue specificityHighly expressed in the erythrocyte, heart and skeletal muscle.
Sequence similaritiesBelongs to the tropomodulin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- D9S57E antibody
- E Tmod antibody
- E tropomodulin antibody
All lanes : Anti-Tropomodulin 1 antibody (ab230276) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse lung lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Paraffin-embedded human heart tissue stained for Tropomodulin 1 using ab230276 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreatic tissue stained for Tropomodulin 1 using ab230276 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230276 has not yet been referenced specifically in any publications.