Overview

  • Product name
    Anti-Tropomodulin 2 antibody
    See all Tropomodulin 2 primary antibodies
  • Description
    Mouse polyclonal to Tropomodulin 2
  • Host species
    Mouse
  • Specificity
    ab67407 is reactive against mammalian transfected lysate
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-351 of Human Tropomodulin 2

  • Positive control
    • Tropomodulin 2 transfected 293T lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 39.6 kDa.

Target

  • Relevance
    Tropomodulin is the actin-capping protein for the slow growing end of filamentous actin. Tropomodulin 2 is a neuronal specific isoform which is the major binding protein to brain tropomyosin in rat.
  • Cellular localization
    Cytoplasm; cytoskeleton
  • Database links
  • Alternative names
    • Tropomodulin 2 antibody
    • MGC39481 antibody
    • N Tmod antibody
    • Neuronal tropomodulin antibody
    • NTMOD antibody
    • TMOD2 antibody
    • tropomodulin 2 (neuronal) antibody
    • tropomodulin 2 neuronal antibody
    see all

Images

  • All lanes : Anti-Tropomodulin 2 antibody (ab67407) at 1/500 dilution

    Lane 1 : Tropomodulin 2 transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Observed band size: 39.6 kDa (why is the actual band size different from the predicted?)

  • Lane 1 : Anti-Tropomodulin 2 antibody (ab67407) at 1/1000 dilution
    Lane 2 : Anti-Tropomodulin 2 antibody [EPR6353] (ab124833) at 1/1000 dilution

    All lanes : N1E-115 mouse neuroblastoma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Polyclonal HRP conjugate at 1/5000 dilution

    See Abreview

References

This product has been referenced in:
  • Gu QH  et al. miR-26a and miR-384-5p are required for LTP maintenance and spine enlargement. Nat Commun 6:6789 (2015). Read more (PubMed: 25858512) »
  • Hu Z  et al. miR-191 and miR-135 are required for long-lasting spine remodelling associated with synaptic long-term depression. Nat Commun 5:3263 (2014). WB, ICC/IF . Read more (PubMed: 24535612) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Murine Neuroblastoma cell line N1E-115)
Specification
Murine Neuroblastoma cell line N1E-115
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma)
Loading amount
20 µg
Specification
N1E-115 mouse neuroblastoma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Loading amount
5 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up