Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6353] to Tropomodulin 2
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-Tropomodulin 2 antibody [EPR6353]
See all Tropomodulin 2 primary antibodies
DescriptionRabbit monoclonal [EPR6353] to Tropomodulin 2
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Tropomodulin 2 (N terminal). The exact sequence is proprietary.
- Fetal brain and SH-SY5Y cell lysates; Human brain tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.28 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
RelevanceTropomodulin is the actin-capping protein for the slow growing end of filamentous actin. Tropomodulin 2 is a neuronal specific isoform which is the major binding protein to brain tropomyosin in rat.
Cellular localizationCytoplasm; cytoskeleton
- Tropomodulin 2 antibody
- MGC39481 antibody
- N Tmod antibody
All lanes : Anti-Tropomodulin 2 antibody [EPR6353] (ab124833) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
ab124833, at 1/250 dilution, staining Tropomodulin 2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124833 has been referenced in 1 publication.
- Zhou Q et al. Key Genes And Pathways Controlled By E2F1 In Human Castration-Resistant Prostate Cancer Cells. Onco Targets Ther 12:8961-8976 (2019). PubMed: 31802906