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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Tropomyosin
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RecombinantRabMAb

Recombinant Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)

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Western blot - Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)
  • Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10302(B)] to Tropomodulin 3
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human Tropomodulin 3 protein (ab130027)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Tropomodulin 3 antibody [EPR10302(B)]
    See all Tropomodulin 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10302(B)] to Tropomodulin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human Tropomodulin 3 (Q9NYL9)

  • Positive control

    • Caco-2, HeLa, HepG2 or Jurkat lysate, HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10302(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly

Associated products

  • Alternative Versions

    • Anti-Tropomodulin 3 antibody [EPR10302(B)] - BSA and Azide free (ab249221)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant Human Tropomodulin 3 protein (ab130027)
    • Caco 2 whole cell lysate (ab3950)
  • Recombinant Protein

    • Recombinant Human Tropomodulin 3 protein (ab130027)

Applications

Our Abpromise guarantee covers the use of ab155968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the tropomodulin family.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession Q9NYL9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 29766 Human
      • Omim: 605112 Human
      • SwissProt: Q9NYL9 Human
      • Unigene: 4998 Human
      • Alternative names

        • OTTHUMP00000162615 antibody
        • Tmod3 antibody
        • TMOD3_HUMAN antibody
        • Tropomodulin 3 (ubiquitous) antibody
        • Tropomodulin 3 ubiquitous antibody
        • Tropomodulin-3 antibody
        • U Tmod antibody
        • U-Tmod antibody
        • Ubiquitous tropomodulin antibody
        • UTmod antibody
        see all

      Images

      • Western blot - Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)
        Western blot - Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)
        All lanes : Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968) at 1/10000 dilution

        Lane 1 : Caco-2 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : HepG2 cell lysate
        Lane 4 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 39 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)
        Immunocytochemistry/ Immunofluorescence - Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)
        Immunofluorescence analysis of HepG2 cells labeling Tropomodulin 3 with ab155968 at a 1/100 dilution.
      • Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)
        Anti-Tropomodulin 3 antibody [EPR10302(B)] (ab155968)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab155968? Please let us know so that we can cite the reference in this datasheet.

      ab155968 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab155968.
      Please use the links above to contact us or submit feedback about this product.

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