Recombinant Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (ab133292)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5159] to Tropomyosin 1 (alpha) + Tropomyosin 3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] -
Description
Rabbit monoclonal [EPR5159] to Tropomyosin 1 (alpha) + Tropomyosin 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Synthetic peptide within Human Tropomyosin 1 (alpha) aa 250-350 (C terminal). The exact sequence is proprietary.
-
Positive control
- Human skeletal muscle, Human heart, HUVEC, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Human muscle tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5159 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 32-41 kDa (predicted molecular weight: 33 kDa).
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 32-41 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Cellular localization
Tropomyosin 1 (alpha): Cytoplasm > cytoskeleton. Tropomyosin 3: Cytoplasm > cytoskeleton. -
Database links
- Entrez Gene: 7168 Human
- Entrez Gene: 7170 Human
- Entrez Gene: 22003 Mouse
- Entrez Gene: 59069 Mouse
- Entrez Gene: 100037999 Pig
- Entrez Gene: 414388 Pig
- Entrez Gene: 117557 Rat
- Entrez Gene: 24851 Rat
see all
Images
-
All lanes : Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (ab133292) at 1/1000 dilution
Lane 1 : MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)
Lane 2 : MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)
Lane 3 : MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)
Lane 4 : GST-tagged Recombinant Human TPM4 protein (Full length)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated)
Predicted band size: 33 kDaBlocking and Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds.
-
Imunohistochemical analysis of paraffin-embedded Human muscle tissue labelling Tropomyosin 1 (alpha) with ab133292 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159] (ab133292) at 1/1000 dilution
Lane 1 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
Lane 2 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 3 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : NIN/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : Human skeletal muscle tissue lysate
Lane 8 : Mouse skeletal muscle tissue lysate
Lane 9 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 32,37 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
The band sizes observed are consistent with PMID: 9168473, PMID: 22965424, PMID: 16765662.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab133292 has been referenced in 4 publications.
- Li L et al. Arecoline suppresses epithelial cell viability by upregulating tropomyosin-1 through the transforming growth factor-ß/Smad pathway. Pharm Biol 58:1244-1251 (2020). PubMed: 33332205
- Gong J et al. Long non-coding RNA CASC2 suppresses pulmonary artery smooth muscle cell proliferation and phenotypic switch in hypoxia-induced pulmonary hypertension. Respir Res 20:53 (2019). PubMed: 30857524
- Binek A et al. Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model. Sci Rep 7:12343 (2017). WB ; Pig . PubMed: 28955040
- Rubel CE et al. Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates. Cell Biochem Biophys 67:127-38 (2013). PubMed: 23695782