Overview

  • Product name
    Anti-Tropomyosin 1 (alpha) antibody [EPR5159]
    See all Tropomyosin 1 (alpha) primary antibodies
  • Description
    Rabbit monoclonal [EPR5159] to Tropomyosin 1 (alpha)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human Tropomyosin 1 (alpha) aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Human skeletal muscle, Human heart, HUVEC, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Human muscle tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32-41 kDa (predicted molecular weight: 33 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
  • Tissue specificity
    Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.
  • Involvement in disease
    Defects in TPM1 are the cause of cardiomyopathy familial hypertrophic type 3 (CMH3) [MIM:115196]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TPM1 are the cause of cardiomyopathy dilated type 1Y (CMD1Y) [MIM:611878]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities
    Belongs to the tropomyosin family.
  • Domain
    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA986836 antibody
    • AI854628 antibody
    • Alpha tropomyosin antibody
    • alpha-TM antibody
    • Alpha-tropomyosin antibody
    • C15orf13 antibody
    • cardiomyopathy, hypertrophic 3 antibody
    • CMD1Y antibody
    • CMH3 antibody
    • HTM alpha antibody
    • HTM-alpha antibody
    • OTTHUMP00000163688 antibody
    • sarcomeric tropomyosin kappa antibody
    • TM2 antibody
    • Tmpa antibody
    • TMSA antibody
    • Tpm-1 antibody
    • TPM1 antibody
    • TPM1_HUMAN antibody
    • tropomyosin 1 (alpha) antibody
    • tropomyosin 1 (alpha) isoform 1 antibody
    • tropomyosin 1 (alpha) isoform 2 antibody
    • tropomyosin 1 (alpha) isoform 3 antibody
    • tropomyosin 1 (alpha) isoform 4 antibody
    • tropomyosin 1 (alpha) isoform 5 antibody
    • tropomyosin 1 (alpha) isoform 6 antibody
    • tropomyosin 1 (alpha) isoform 7 antibody
    • Tropomyosin 1 antibody
    • Tropomyosin alpha 1 chain antibody
    • Tropomyosin alpha-1 chain antibody
    • Tropomyosin, skeletal muscle alpha antibody
    • Tropomyosin-1 antibody
    see all

Images

  • Imunohistochemical analysis of paraffin-embedded Human muscle tissue labelling Tropomyosin 1 (alpha) with ab133292 at 1/100 dilution.
  • All lanes : Anti-Tropomyosin 1 (alpha) antibody [EPR5159] (ab133292) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human heart lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : PC12 cell lysate
    Lane 7 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 33 kDa

References

This product has been referenced in:
  • Binek A  et al. Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model. Sci Rep 7:12343 (2017). WB ; Pig . Read more (PubMed: 28955040) »
  • Rubel CE  et al. Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates. Cell Biochem Biophys 67:127-38 (2013). Read more (PubMed: 23695782) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (Embryonic heart)
Specification
Embryonic heart
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 30 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4%-12%)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 30 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Total protein in input
150 µg

Abcam user community

Verified customer

Submitted Dec 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up