Overview

  • Product name

    Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal
    See all Tropomyosin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12846] to Tropomyosin 2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Tropomyosin 2 aa 200 to the C-terminus (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: P07951

  • Positive control

    • HeLa, HT-1080, fetal muscle and heart lysates, Human fetal muscle lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12846
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.
    • Tissue specificity

      Present in primary breast cancer tissue, absent from normal breast tissue.
    • Involvement in disease

      Nemaline myopathy 4 (NEM4) [MIM:609285]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Note=The disease is caused by mutations affecting the gene represented in this entry.
      Arthrogryposis, distal, 1A (DA1A) [MIM:108120]: A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • Sequence similarities

      Belongs to the tropomyosin family.
    • Domain

      The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
    • Post-translational
      modifications

      Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha tropomyosin antibody
      • AMCD1 antibody
      • Arthrogryposis multiplex congenital distal type 1 antibody
      • Beta tropomyosin antibody
      • Beta-tropomyosin antibody
      • Cytoskeletal tropomyosin TM30 antibody
      • DA1 antibody
      • DA2B antibody
      • epididymis secretory protein Li 273 antibody
      • FLJ41118 antibody
      • Heat stable cytoskeletal protein 30 kDa antibody
      • HEL-S-273 antibody
      • hscp30 antibody
      • HTM alpha antibody
      • hTM5 antibody
      • MGC14582 antibody
      • MGC3261 antibody
      • MGC72094 antibody
      • NEM1 antibody
      • NEM4 antibody
      • Nemaline myopathy type 4 antibody
      • OK/SW cl.5 antibody
      • Sarcomeric tropomyosin kappa antibody
      • TM 5 antibody
      • TM3 antibody
      • TM30 antibody
      • TM30nm antibody
      • TMSA antibody
      • TMSB antibody
      • TPM 1 antibody
      • TPM 3 antibody
      • TPM1 alpha antibody
      • TPM1 kappa antibody
      • TPM2 antibody
      • TPM2_HUMAN antibody
      • TRK antibody
      • Tropomyosin 1 alpha antibody
      • Tropomyosin 1 alpha chain antibody
      • Tropomyosin 1 alpha chain isoform 6 antibody
      • Tropomyosin 2 (beta) antibody
      • Tropomyosin 2 antibody
      • Tropomyosin 3 antibody
      • Tropomyosin alpha 3 chain antibody
      • Tropomyosin alpha striated muscle isoform antibody
      • Tropomyosin beta chain antibody
      • Tropomyosin gamma antibody
      • Tropomyosin skeletal muscle beta antibody
      • Tropomyosin-2 antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate labeling Tropomyosin 2 using ab180176 at 1/10.

    • All lanes : Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HT-1080 cell lysate
      Lane 4 : Fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 33 kDa

    References

    This product has been referenced in:

    • Gao M & Si X Rapamycin ameliorates psoriasis by regulating the expression and methylation levels of tropomyosin via ERK1/2 and mTOR pathways in vitro and in vivo. Exp Dermatol 27:1112-1119 (2018). Read more (PubMed: 30019485) »
    See 1 Publication for this product

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