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  1. Link

    tropomyosin-2-antibody-epr12846-c-terminal-ab180176.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Tropomyosin
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RecombinantRabMAb

Recombinant Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)

  • Datasheet
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Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
  • Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
  • Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
  • Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12846] to Tropomyosin 2 - C-terminal
  • Suitable for: IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human Tropomyosin 2 protein (ab103503)
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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal
    See all Tropomyosin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12846] to Tropomyosin 2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Tropomyosin 2 aa 200 to the C-terminus (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: P07951

  • Positive control

    • WB: HeLa, HT-1080, fetal muscle and heart lysates, Human fetal muscle lysate. MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12846
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin

Associated products

  • Alternative Versions

    • Anti-Tropomyosin 2 antibody [EPR12846] - BSA and Azide free (ab250178)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Tropomyosin 2 protein (ab103503)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/10 - 1/100.
WB
1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
Notes
IP
1/10 - 1/100.
WB
1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.
  • Tissue specificity

    Present in primary breast cancer tissue, absent from normal breast tissue.
  • Involvement in disease

    Nemaline myopathy 4 (NEM4) [MIM:609285]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Arthrogryposis, distal, 1A (DA1A) [MIM:108120]: A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the tropomyosin family.
  • Domain

    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Post-translational
    modifications

    Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P07951 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7169 Human
    • Entrez Gene: 22004 Mouse
    • Entrez Gene: 500450 Rat
    • Omim: 190990 Human
    • SwissProt: P07951 Human
    • SwissProt: P58774 Mouse
    • SwissProt: P58775 Rat
    • Unigene: 300772 Human
    • Unigene: 646 Mouse
    • Unigene: 17580 Rat
    see all
  • Alternative names

    • Alpha tropomyosin antibody
    • AMCD1 antibody
    • Arthrogryposis multiplex congenital distal type 1 antibody
    • Beta tropomyosin antibody
    • Beta-tropomyosin antibody
    • Cytoskeletal tropomyosin TM30 antibody
    • DA1 antibody
    • DA2B antibody
    • epididymis secretory protein Li 273 antibody
    • FLJ41118 antibody
    • Heat stable cytoskeletal protein 30 kDa antibody
    • HEL-S-273 antibody
    • hscp30 antibody
    • HTM alpha antibody
    • hTM5 antibody
    • MGC14582 antibody
    • MGC3261 antibody
    • MGC72094 antibody
    • NEM1 antibody
    • NEM4 antibody
    • Nemaline myopathy type 4 antibody
    • OK/SW cl.5 antibody
    • Sarcomeric tropomyosin kappa antibody
    • TM 5 antibody
    • TM3 antibody
    • TM30 antibody
    • TM30nm antibody
    • TMSA antibody
    • TMSB antibody
    • TPM 1 antibody
    • TPM 3 antibody
    • TPM1 alpha antibody
    • TPM1 kappa antibody
    • TPM2 antibody
    • TPM2_HUMAN antibody
    • TRK antibody
    • Tropomyosin 1 alpha antibody
    • Tropomyosin 1 alpha chain antibody
    • Tropomyosin 1 alpha chain isoform 6 antibody
    • Tropomyosin 2 (beta) antibody
    • Tropomyosin 2 antibody
    • Tropomyosin 3 antibody
    • Tropomyosin alpha 3 chain antibody
    • Tropomyosin alpha striated muscle isoform antibody
    • Tropomyosin beta chain antibody
    • Tropomyosin gamma antibody
    • Tropomyosin skeletal muscle beta antibody
    • Tropomyosin-2 antibody
    see all

Images

  • Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
    Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
    All lanes : Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176) at 1/1000 dilution

    Lane 1 : MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)
    Lane 2 : MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)
    Lane 3 : MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)
    Lane 4 : GST-tagged Recombinant Human TPM4 protein (Full length)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
    Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)

    Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate labeling Tropomyosin 2 using ab180176 at 1/10.

  • Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
    Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
    All lanes : Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176) at 1/1000 dilution

    Lane 1 : Fetal heart lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HT-1080 cell lysate
    Lane 4 : Fetal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa

  • Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)
    Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab180176? Please let us know so that we can cite the reference in this datasheet.

ab180176 has been referenced in 1 publication.

  • Gao M & Si X Rapamycin ameliorates psoriasis by regulating the expression and methylation levels of tropomyosin via ERK1/2 and mTOR pathways in vitro and in vivo. Exp Dermatol 27:1112-1119 (2018). PubMed: 30019485

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