• Product name
    Anti-Tropomyosin antibody [MAC 141]
    See all Tropomyosin primary antibodies
  • Description
    Rat monoclonal [MAC 141] to Tropomyosin
  • Host species
  • Specificity
    Antibody reacts with tropomyosin in Lethocerus and Drosophila flight and non-flight muscle and with tropomyosin in flight muscles of all other insect species tested. Antibody reacts with Troponin H (TnH) in Lethocerus flight muscle, the 33 and 34 isoforms of TnH in Drosophila and proteins of TnH molecular weight in flight muscles of other Diptera and Lepidoptera. Antibody also reacts with tropomyosin in muscles of Crustacea and Caenorhabditis (nematode).
  • Tested applications
    Suitable for: WB, IHC-Fr, Electron Microscopy, ICC/IF, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster, Waterbug
    Predicted to work with: Caenorhabditis elegans, A wide range of other invertebrates
  • Immunogen

    Troponin-tropomyosin complex purified from Lethocerus indicus (Waterbug) flight muscle.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Clone number
    MAC 141
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab50567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 19272319
Electron Microscopy Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration. PubMed: 22841645


  • Relevance
    Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
  • Cellular localization
  • Alternative names
    • Alpha tropomyosin antibody
    • Beta tropomyosin antibody
    • TM30nm antibody
    • TPM1 antibody
    • TPM2 antibody
    • TPM3 antibody
    • Tropomyosin 1 alpha antibody
    • Tropomyosin 1 antibody
    • Tropomyosin 2 antibody
    • Tropomyosin 2 beta antibody
    • Tropomyosin 3 antibody
    see all


  • Immunohistochemical analysis of wholemount Drosohopila melanogaster embryo, staining Tropomyosin with ab50567 at 1/1000 dilution.
  • Immunohistochemical analysis of wholemount Drosohopila melanogaster embryo, staining Tropomyosin with ab50567 at 1/1000 dilution.


This product has been referenced in:
  • Camuglia JM  et al. An RNAi based screen in Drosophila larvae identifies fascin as a regulator of myoblast fusion and myotendinous junction structure. Skelet Muscle 8:12 (2018). Read more (PubMed: 29625624) »
  • Boukhatmi H & Bray S A population of adult satellite-like cells in Drosophila is maintained through a switch in RNA-isoforms. Elife 7:N/A (2018). Read more (PubMed: 29629869) »
See all 20 Publications for this product

Customer reviews and Q&As


Thank you for your enquiry. I have been informed by the source of this product of the following information: " In 1993 Dr Matthew Colloff (Dept Bacteriology, Stobhill General Hospital, Glasgow) said that MAC 141 reacts with prawn tropomyosin. He found prawn tropomyosin is a major allergen of prawns in humans. He also found actin is an allergen using MAC237. His publications can be found in PubMed." We would be delighted to add your publication to our datasheet, please do let us know the Pubmed ID and also consider submitting an Abreview via the online datasheet whereby we will reward your feedback (positive or negative) with Abpoints exchangeable for discounts off your future orders.

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