Product nameAnti-Tropomyosin antibody [MAC 141]
See all Tropomyosin primary antibodies
DescriptionRat monoclonal [MAC 141] to Tropomyosin
SpecificityAntibody reacts with tropomyosin in Lethocerus and Drosophila flight and non-flight muscle and with tropomyosin in flight muscles of all other insect species tested. Antibody reacts with Troponin H (TnH) in Lethocerus flight muscle, the 33 and 34 isoforms of TnH in Drosophila and proteins of TnH molecular weight in flight muscles of other Diptera and Lepidoptera. Antibody also reacts with tropomyosin in muscles of Crustacea and Caenorhabditis (nematode).
Tested applicationsSuitable for: WB, IHC-Fr, Electron Microscopy, ICC/IF, IHC - Wholemountmore details
Species reactivityReacts with: Drosophila melanogaster, Waterbug
Predicted to work with: Caenorhabditis elegans, A wide range of other invertebrates
Troponin-tropomyosin complex purified from Lethocerus indicus (Waterbug) flight muscle.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Clone numberMAC 141
Our Abpromise guarantee covers the use of ab50567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19272319|
|Electron Microscopy||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration. PubMed: 22841645|
RelevanceTropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
- Alpha tropomyosin antibody
- Beta tropomyosin antibody
- TM30nm antibody
This product has been referenced in:
- Camuglia JM et al. An RNAi based screen in Drosophila larvae identifies fascin as a regulator of myoblast fusion and myotendinous junction structure. Skelet Muscle 8:12 (2018). Read more (PubMed: 29625624) »
- Boukhatmi H & Bray S A population of adult satellite-like cells in Drosophila is maintained through a switch in RNA-isoforms. Elife 7:N/A (2018). Read more (PubMed: 29629869) »