Anti-Tropomyosin antibody [TM-311] (ab7785)
Key features and details
- Mouse monoclonal [TM-311] to Tropomyosin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Tropomyosin antibody [TM-311] -
Description
Mouse monoclonal [TM-311] to Tropomyosin -
Host species
Mouse -
Specificity
This antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified chicken gizzard tropomyosin.
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Positive control
- WB: Human heart tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
TM-311 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa).
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Notes |
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WB
1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa). |
Target
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Relevance
Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 7168 Human
- Entrez Gene: 7169 Human
- Entrez Gene: 7170 Human
- Omim: 190990 Human
- Omim: 191010 Human
- Omim: 191030 Human
- SwissProt: P06753 Human
- SwissProt: P07951 Human
see all -
Alternative names
- Alpha tropomyosin antibody
- Beta tropomyosin antibody
- TM30nm antibody
see all
Images
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Anti-Tropomyosin antibody [TM-311] (ab7785) at 1/200 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab7785 has been referenced in 15 publications.
- Zhang W et al. Skullcapflavone I inhibits proliferation of human colorectal cancer cells via down-regulation of miR-107 expression. Neoplasma 66:203-210 (2019). PubMed: 30509103
- Shibata T et al. Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity. Mech Ageing Dev 171:24-30 (2018). IHC-P ; Mouse . PubMed: 29510160
- Sui Z et al. Stabilization of F-actin by tropomyosin isoforms regulates the morphology and mechanical behavior of red blood cells. Mol Biol Cell 28:2531-2542 (2017). PubMed: 28720661
- Ijaz F et al. Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires Ttll4. Mol Biol Cell 28:535-544 (2017). PubMed: 27974641
- Ichimura T et al. Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging. Sci Rep 5:11358 (2015). WB ; Mouse . PubMed: 26079396