Overview

  • Product name
    Anti-Tropomyosin antibody [TM-311]
    See all Tropomyosin primary antibodies
  • Description
    Mouse monoclonal [TM-311] to Tropomyosin
  • Host species
    Mouse
  • Specificity
    This antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Purified chicken gizzard tropomyosin.

  • Positive control
    • WB: Human heart tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    TM-311
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa).

Target

Images

  • Anti-Tropomyosin antibody [TM-311] (ab7785) at 1/200 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Shibata T  et al. Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity. Mech Ageing Dev 171:24-30 (2018). IHC-P ; Mouse . Read more (PubMed: 29510160) »
  • Sui Z  et al. Stabilization of F-actin by tropomyosin isoforms regulates the morphology and mechanical behavior of red blood cells. Mol Biol Cell 28:2531-2542 (2017). Read more (PubMed: 28720661) »

See all 12 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Myometrium)
Loading amount
20 µg
Specification
Myometrium
Gel Running Conditions
Reduced Denaturing (gel 12%,)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 14 2011

Thank you for your phone call. The originator of ab7785 provided me with the following information regarding this antibody. The molecular weight of tropomyosin is 70 kDa. The alpha isomer is 36 kDa and the beta isomer is 35 kDa. In electrophoresis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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