Antibody reacts with Troponin H (TnH) in Lethocerus flight muscle, the 34 isoform of TnH in Drosophila and proteins of TnH molecular weight in flight muscle of all other insect species tested. Antibody also reacts with zeelin 2 in Lethocerus and glutathione-S-transferase-2 kin Drosophila. Antibody reacts with Drosophila BiP (hsc72).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Troponin H (TnH) is a tropomyosin isoform found in insect indirect flight muscle. It has a C-terminal extension that connects to the myosin cross-bridge. Two isoforms of TnH exist in Drosophila, at 33 and 34 kDa.
Kulke M et al. Kettin, a major source of myofibrillar stiffness in Drosophila indirect flight muscle. J Cell Biol154:1045-57 (2001).
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Clayton JD et al. Interaction of troponin-H and glutathione S-transferase-2 in the indirect flight muscles of Drosophila melanogaster. J Muscle Res Cell Motil19:117-27 (1998).
Read more (PubMed: 9536439) »