Anti-Troponin I fast skeletal muscle antibody (ab134838)
Key features and details
- Rabbit polyclonal to Troponin I fast skeletal muscle
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Troponin I fast skeletal muscle antibody
See all Troponin I fast skeletal muscle primary antibodies -
Description
Rabbit polyclonal to Troponin I fast skeletal muscle -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide, corresponding to residues in Human Troponin I fast skeletal muscle.
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Positive control
- Human Skeletal Muscle tissue; Human brain lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/200 - 1/500. Predicted molecular weight: 21 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/200 - 1/500. Predicted molecular weight: 21 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. -
Sequence similarities
Belongs to the troponin I family. - Information by UniProt
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Database links
- Entrez Gene: 7136 Human
- Entrez Gene: 21953 Mouse
- Entrez Gene: 29389 Rat
- Omim: 191043 Human
- SwissProt: P48788 Human
- SwissProt: P13412 Mouse
- SwissProt: P27768 Rat
- Unigene: 523403 Human
see all -
Alternative names
- AMCD 2B antibody
- AMCD2B antibody
- DA 2B antibody
see all
Images
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Western blot - Anti-Troponin I fast skeletal muscle antibody (ab134838)Anti-Troponin I fast skeletal muscle antibody (ab134838) at 1/200 dilution + Human Brain lysate
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Troponin I fast skeletal muscle antibody (ab134838)Immunohistochemistry analysis of formalin fixed, paraffin embedded Human Skeletal Muscle tissue labelling Troponin I fast skeletal muscle with ab134838 at 5 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134838 has been referenced in 3 publications.
- Morisasa M et al. Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging. J Oleo Sci 68:141-148 (2019). PubMed: 30713267
- De Matteis A et al. The Troponin-I fast skeletal muscle is reliable marker for the determination of vitality in the suicide hanging. Forensic Sci Int 301:284-288 (2019). PubMed: 31195249
- Ponnusamy S et al. Androgen receptor agonists increase lean mass, improve cardiopulmonary functions and extend survival in preclinical models of Duchenne muscular dystrophy. Hum Mol Genet 26:2526-2540 (2017). PubMed: 28453658