Recombinant
RabMAb

Recombinant Anti-Troponin I fast skeletal muscle antibody [EPR14926] (ab183508)

Overview

  • Product name

    Anti-Troponin I fast skeletal muscle antibody [EPR14926]
    See all Troponin I fast skeletal muscle primary antibodies
  • Description

    Rabbit monoclonal [EPR14926] to Troponin I fast skeletal muscle
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Troponin I fast skeletal muscle aa 1-100. The exact sequence is proprietary.
    Database link: P48788

  • Positive control

    • Human skeletal muscle lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14926
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/100000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease

    Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.
  • Sequence similarities

    Belongs to the troponin I family.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMCD 2B antibody
    • AMCD2B antibody
    • DA 2B antibody
    • DA2B antibody
    • fast skeletal muscle antibody
    • Fast twitch skeletal muscle troponin I antibody
    • fast-twitch isoform antibody
    • FSSV antibody
    • fsTnI antibody
    • TNNI 2 antibody
    • Tnni2 antibody
    • TNNI2_HUMAN antibody
    • tro antibody
    • Troponin I antibody
    • Troponin I fast skeletal muscle antibody
    • Troponin I fast twitch 2 antibody
    • Troponin I fast twitch isoform antibody
    • Troponin I fast twitch skeletal muscle isoform antibody
    • Troponin I skeletal fast antibody
    • Troponin I type 2 (skeletal fast) antibody
    • Troponin I type 2 antibody
    see all

Images

  • Anti-Troponin I fast skeletal muscle antibody [EPR14926] (ab183508) at 1/100000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.

References

ab183508 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryonic cardiac tissue)
Permeabilization
Yes - sodium citrate antigen retrieval
Specification
embryonic cardiac tissue
Blocking step
FBS and 1% TritonX100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 14 2016

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