Recombinant Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14927(B)] to Troponin I fast skeletal muscle
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)]
See all Troponin I fast skeletal muscle primary antibodies -
Description
Rabbit monoclonal [EPR14927(B)] to Troponin I fast skeletal muscle -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle, mouse muscle and rat muscle lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14927(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/20.
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WB |
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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Notes |
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IP
1/20. |
WB
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. -
Sequence similarities
Belongs to the troponin I family. - Information by UniProt
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Database links
- Entrez Gene: 7136 Human
- Entrez Gene: 21953 Mouse
- Entrez Gene: 29389 Rat
- Omim: 191043 Human
- SwissProt: P48788 Human
- SwissProt: P13412 Mouse
- SwissProt: P27768 Rat
- Unigene: 523403 Human
see all -
Alternative names
- AMCD 2B antibody
- AMCD2B antibody
- DA 2B antibody
see all
Images
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Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
All lanes : Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Western blot analysis of Troponin I fast skeletal muscle in Human skeletal muscle lysate immunoprecipitated using ab184554 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human skeletal muscle lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab184554 has been referenced in 3 publications.
- Fu Z et al. SYT8 promotes pancreatic cancer progression via the TNNI2/ERRα/SIRT1 signaling pathway. Cell Death Discov 7:390 (2021). PubMed: 34907162
- Teigen LE et al. Ca2+ dependency of limb muscle fiber contractile mechanics in young and older adults. Am J Physiol Cell Physiol 318:C1238-C1251 (2020). PubMed: 32348175
- Wang S et al. Long noncoding RNA Neat1 modulates myogenesis by recruiting Ezh2. Cell Death Dis 10:505 (2019). PubMed: 31243262