Recombinant Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] - BSA and Azide free (ab250756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14927(B)] to Troponin I fast skeletal muscle - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] - BSA and Azide free
See all Troponin I fast skeletal muscle primary antibodies -
Description
Rabbit monoclonal [EPR14927(B)] to Troponin I fast skeletal muscle - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250756 is the carrier-free version of ab184554.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14927(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Involvement in disease
Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. -
Sequence similarities
Belongs to the troponin I family. - Information by UniProt
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Database links
- Entrez Gene: 7136 Human
- Entrez Gene: 21953 Mouse
- Entrez Gene: 29389 Rat
- Omim: 191043 Human
- SwissProt: P48788 Human
- SwissProt: P13412 Mouse
- SwissProt: P27768 Rat
- Unigene: 523403 Human
see all -
Alternative names
- AMCD 2B antibody
- AMCD2B antibody
- DA 2B antibody
see all
Images
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Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaThis data was developed using ab184554, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaThis data was developed using ab184554, the same antibody clone in a different buffer formulation.
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This data was developed using ab184554, the same antibody clone in a different buffer formulation.Western blot analysis of Troponin I fast skeletal muscle in Human skeletal muscle lysate immunoprecipitated using ab184554 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human skeletal muscle lysate.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250756 has not yet been referenced specifically in any publications.