Overview

  • Product name
  • Description
    Rabbit polyclonal to Troponin T1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-278 of Human Troponin T1 (UniProt ID: P13805).

  • Positive control
    • H1299 whole cell lysate. Human colon carcinoma and pig skeletal muscle tissue.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease
    Nemaline myopathy 5
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANM antibody
    • MGC104241 antibody
    • NEM5 antibody
    • Skeletal antibody
    • Slow antibody
    • slow skeletal muscle antibody
    • Slow skeletal muscle troponin T antibody
    • sTnT antibody
    • Tnnt1 antibody
    • TNNT1_HUMAN antibody
    • TNT antibody
    • TnTs antibody
    • Troponin T antibody
    • Troponin T slow skeletal muscle antibody
    • Troponin T type 1 (skeletal slow) antibody
    • troponin T1 skeletal slow antibody
    • Troponin T1, slow skeletal type antibody
    see all

Images

  • Anti-Troponin T1 antibody (ab155028) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 33 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue, labeling Troponin T1 using ab155028 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin embedded Pig skeletal muscle tissue, labeling Troponin T1 using ab155028 at 1/500 dilution.

References

ab155028 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Fetal skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate PH 6.0
Specification
Fetal skeletal muscle
Blocking step
Horse serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Ms. Serene Joseph

Verified customer

Submitted Mar 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up