Overview

  • Product name

    Anti-Troponin T1/TNT antibody
    See all Troponin T1/TNT primary antibodies
  • Description

    Rabbit polyclonal to Troponin T1/TNT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Troponin T1/TNT aa 228-277 (internal sequence).
    Database link: NP_003274

  • Positive control

    • Human fetal muscle lysate.
  • General notes

     This product was previously labelled as Troponin T1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in a peptide based assay is 1/312500.

Target

Images

  • Anti-Troponin T1/TNT antibody (ab83907) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.



    12% gel

References

This product has been referenced in:

  • Hyzewicz J  et al. Low-Intensity Training and the C5a Complement Antagonist NOX-D21 Rescue the mdx Phenotype through Modulation of Inflammation. Am J Pathol 187:1147-1161 (2017). Read more (PubMed: 28315675) »
See 1 Publication for this product

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