Product nameAnti-TROY antibody [EPR3213(2)]
See all TROY primary antibodies
DescriptionRabbit monoclonal [EPR3213(2)] to TROY
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TROY aa 150-250. The exact sequence is proprietary.
- Human prostate cancer, A431, LnCaP, PC3, U87-MG, and fetal brain lysates, Human prostate tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab138502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCan mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death.
Tissue specificityHighly expressed in prostate. Detected at lower levels in thymus, spleen, testis, uterus, small intestine, colon and peripheral blood leukocytes.
Sequence similaritiesContains 3 TNFR-Cys repeats.
- Information by UniProt
- AL023044 antibody
- AW123854 antibody
- TAJ alpha antibody
All lanes : Anti-TROY antibody [EPR3213(2)] (ab138502) at 1/1000 dilution
Lane 1 : Human prostate
Lane 2 : A431 lysate
Lane 3 : LnCaP lysate
Lane 4 : PC3 lysate
Lane 5 : U87-MG lysate
Lane 6 : fetal brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Overlay histogram showing PC3 cells stained with ab138502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab138502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical analysis of paraffin embedded Human prostate tissue labelling TROY with ab138502 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Saberi S et al. Immunohistochemical Analysis of LGR5 and TROY Expression in Gastric Carcinogenesis Demonstrates an Inverse Trend Iran Biomed J 23:107-20 (2019). Read more (PubMed: 30501144) »
- Zou Y et al. Overexpression of ubiquitin specific proteases 44 promotes the malignancy of glioma by stabilizing tumor-promoter securin. Oncotarget 8:58231-58246 (2017). Read more (PubMed: 28938551) »