Overlay histogram showing PC3 cells stained with ab138502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab138502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-TROY antibody [EPR3213(2)] (ab138502)
All lanes : Anti-TROY antibody [EPR3213(2)] (ab138502) at 1/1000 dilution
Lane 1 : Human prostate cancer lysate Lane 2 : A431 lysate Lane 3 : LnCaP lysate Lane 4 : PC3 lysate Lane 5 : U87-MG lysate Lane 6 : fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution