Key features and details
- Mouse monoclonal [N64A/36] to TRP 7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-TRP 7 antibody [N64A/36]
DescriptionMouse monoclonal [N64A/36] to TRP 7
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Rat brain lysate
The clone number has been updated from S64A-36 to N64A/36, both clone numbers name the same antibody clone.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) (By similarity). May also be activated by intracellular calcium store depletion.
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC7 sub-subfamily.
Contains 4 ANK repeats.
- Information by UniProt
- Capacitative calcium channel TRPC7 antibody
- cation channel, subfamily C, member 7 antibody
- hTRP7 antibody
Western blot detection of Trp7 in Rat brain membrane lysates using ab93618 at 1:1000 dilution.
Cold 100% methanol for 10 minutes at -20°C fixed HaCaT cells labeling TRP 7 (green) using ab93618 at 1/100 dilution for 1 hour at room temperature. FITC Goat anti-mouse (green) was used as the secondary antibody at 1/50 dilution for 1 hour at room temperature. Nuclear staining is observed.
Bouin's-fixed, paraffin-embedded mouse backskin tissue stained for TRP 7 (green) using ab93618 at 1/100 dilution in immunohistochemical analysis. Goat Anti-Mouse was used as the secodary antibody at 1/50 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93618 has been referenced in 1 publication.
- Nakipova OV et al. Store-operated Ca2+ entry supports contractile function in hearts of hibernators. PLoS One 12:e0177469 (2017). PubMed: 28531217