Recombinant
RabMAb

Recombinant Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

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Overview

  • Product name

    Anti-TRP1 antibody [EPR13064(B)] - C-terminal
    See all TRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13064(B)] to TRP1 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human TRP1 (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P17643

  • Positive control

    • Human melanoma and Human skin lysates. Human melanoma tissue and Human skin tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.

    • Tissue specificity

      Pigment cells.

    • Pathway

      Pigment biosynthesis; melanin biosynthesis.

    • Involvement in disease

      Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.

    • Sequence similarities

      Belongs to the tyrosinase family.

    • Cellular localization

      Melanosome membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 5 antibody
        • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
        • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
        • b-PROTEIN antibody
        • CAS2 antibody
        • Catalase B antibody
        • CATB antibody
        • DHICA oxidase antibody
        • Glycoprotein 75 antibody
        • GP75 antibody
        • Melanoma antigen gp75 antibody
        • OCA3 antibody
        • TRP antibody
        • TRP-1 antibody
        • TRP1 antibody
        • Tyrosinase related protein 1 antibody
        • Tyrosinase-related protein 1 antibody
        • TYRP antibody
        • TYRP1 antibody
        • TYRP1_HUMAN antibody
        • TYRRP antibody
        see all

      Images

      • Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        All lanes : Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468) at 1/1000 dilution

        Lane 1 : Human melanoma lysate
        Lane 2 : Human skin lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 60 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

        Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling TRP1 with ab177468 at 1/50 diultion.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

        Western blot analysis on immunoprecipitation pellet from fetal skin lysate using ab177468 at 1/10 dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

        Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab177468 at 1/50 diultion.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      ab177468 has not yet been referenced specifically in any publications.

      Publishing research using ab177468? Please let us know so that we can cite the reference in this datasheet.

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