Recombinant Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
![Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)](https://www.abcam.com/ps/products/177/ab177468/Images/ab177468-205865-anti-trp1-antibody-epr13064b-c-terminal-western-blot.jpg "Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13064(B)] to TRP1 - C-terminal
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-TRP1 antibody [EPR13064(B)] - C-terminal
See all TRP1 primary antibodies -
Description
Rabbit monoclonal [EPR13064(B)] to TRP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human TRP1 (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P17643 -
Positive control
- Human melanoma and Human skin lysates. Human melanoma tissue and Human skin tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13064(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 60 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | 1/10 - 1/100. |
Target
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Function
Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. -
Tissue specificity
Pigment cells. -
Pathway
Pigment biosynthesis; melanin biosynthesis. -
Involvement in disease
Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. -
Sequence similarities
Belongs to the tyrosinase family. -
Cellular localization
Melanosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7306 Human
- Omim: 115501 Human
- SwissProt: P17643 Human
- Unigene: 270279 Human
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Alternative names
- 5 antibody
- 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
- 6-dihydroxyindole-2-carboxylic acid oxidase antibody
see all
Images
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Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)All lanes : Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468) at 1/1000 dilution
Lane 1 : Human melanoma lysate
Lane 2 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)](https://www.abcam.com/ps/products/177/ab177468/Images/ab177468-205866-anti-trp1-antibody-epr13064b-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling TRP1 with ab177468 at 1/50 diultion.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)](https://www.abcam.com/ps/products/177/ab177468/Images/ab177468-205868-anti-trp1-antibody-epr13064b-c-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)Western blot analysis on immunoprecipitation pellet from fetal skin lysate using ab177468 at 1/10 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)](https://www.abcam.com/ps/products/177/ab177468/Images/ab177468-205867-anti-trp1-antibody-epr13064b-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab177468 at 1/50 diultion.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)](https://www.abcam.com/ps/products/177/ab177468/Images/ab177468-5-benefits-of-recombinant-antibodies.png)
Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
Protocols
Datasheets and documents
References (0)
ab177468 has not yet been referenced specifically in any publications.
