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RecombinantRabMAb

Recombinant Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

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Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
  • Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
  • Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13064(B)] to TRP1 - C-terminal
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

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Recombinant Human TRP1 protein (His tag) (ab219285)

View more associated products

Overview

  • Product name

    Anti-TRP1 antibody [EPR13064(B)] - C-terminal
    See all TRP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13064(B)] to TRP1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human TRP1 (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P17643

  • Positive control

    • Human melanoma and Human skin lysates. Human melanoma tissue and Human skin tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13064(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Alternative Versions

    • Anti-TRP1 antibody [EPR13064(B)] - BSA and Azide free (ab249974)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human TRP1 protein (His tag) (ab219285)
  • Related Products

    • Recombinant Human TRP1 protein (ab132102)

Applications

Our Abpromise guarantee covers the use of ab177468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
    • Tissue specificity

      Pigment cells.
    • Pathway

      Pigment biosynthesis; melanin biosynthesis.
    • Involvement in disease

      Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
    • Sequence similarities

      Belongs to the tyrosinase family.
    • Cellular localization

      Melanosome membrane.
    • Target information above from: UniProt accession P17643 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7306 Human
      • Omim: 115501 Human
      • SwissProt: P17643 Human
      • Unigene: 270279 Human
      • Alternative names

        • 5 antibody
        • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
        • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
        • b-PROTEIN antibody
        • CAS2 antibody
        • Catalase B antibody
        • CATB antibody
        • DHICA oxidase antibody
        • Glycoprotein 75 antibody
        • GP75 antibody
        • Melanoma antigen gp75 antibody
        • OCA3 antibody
        • TRP antibody
        • TRP-1 antibody
        • TRP1 antibody
        • Tyrosinase related protein 1 antibody
        • Tyrosinase-related protein 1 antibody
        • TYRP antibody
        • TYRP1 antibody
        • TYRP1_HUMAN antibody
        • TYRRP antibody
        see all

      Images

      • Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Western blot - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        All lanes : Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468) at 1/1000 dilution

        Lane 1 : Human melanoma lysate
        Lane 2 : Human skin lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 60 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

        Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling TRP1 with ab177468 at 1/50 diultion.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Immunoprecipitation - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

        Western blot analysis on immunoprecipitation pellet from fetal skin lysate using ab177468 at 1/10 dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

        Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab177468 at 1/50 diultion.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
        Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab177468? Please let us know so that we can cite the reference in this datasheet.

      ab177468 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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