This monoclonal antibody detects a differentiation related, pigmentation-associated glycoprotein (gp75), expressed by melanoma cells, nomal melanocytes and nevi. This antibody does not react with carcinomas and
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For optimal staining, the primary antibody should be incubated 20-60 minutes at room temperature.
Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
Pigment biosynthesis; melanin biosynthesis.
Involvement in disease
Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.