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Synthetic peptide corresponding to Human TRP2/ DCT (internal sequence). synthesized peptide derived from internal of human TRP2.
Previously labelled as TRP2
Our Abpromise guarantee covers the use of ab74073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 59 kDa.
TRP2 protein is a single-pass type I membrane protein, these types of proteins known to aggregate when boiled therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70C instead of boiling.
|ICC/IF||1/100 - 1/500.|
ab74043 staining TRP2 / DCT in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using a TRIS/EDTA buffer pH 9.0. Samples were then permeabilized, followed by incubation with the primary antibody at a 1/1000 dilution for 30 minutes at 20°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
ab74073 staining TRP2 / DCT in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunofluorescence analysis of TRP2 / DCT in A549 cells, using ab74073 at a dilution of 1/500.
Left hand panel: no peptide
right hand panel: with immunising peptide.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"