Overview

  • Product name
  • Description
    Rabbit polyclonal to TRP2 / DCT
  • Host species
    Rabbit
  • Specificity
    ab74073 detects endogenous levels of total TRP2 / DCT protein.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRP2/ DCT (internal sequence). synthesized peptide derived from internal of human TRP2.

  • Positive control
    • WB: Extracts from K562 cells ICC/IF: A549 cells
  • General notes

    Previously labelled as TRP2

Properties

Applications

Our Abpromise guarantee covers the use of ab74073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.

TRP2 protein is a single-pass type I membrane protein, these types of proteins known to aggregate when boiled therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70C instead of boiling.   

ELISA 1/40000.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Involved in regulating eumelanin and phaeomelanin levels.
  • Pathway
    Pigment biosynthesis; melanin biosynthesis.
  • Sequence similarities
    Belongs to the tyrosinase family.
  • Cellular localization
    Melanosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • L dopachrome tautomerase antibody
    • DCT antibody
    • Dopachrome delta isomerase antibody
    • Dopachrome tautomerase antibody
    • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
    • DT antibody
    • EC 5.3.3.12 antibody
    • L dopachrome Delta isomerase antibody
    • L-dopachrome Delta-isomerase antibody
    • L-dopachrome tautomerase antibody
    • TRP 2 antibody
    • TRP-2 antibody
    • TRP2 antibody
    • Tyrosinase related protein 2 antibody
    • Tyrosinase-related protein 2 antibody
    • TYRP2 antibody
    • TYRP2_HUMAN antibody
    see all

Images

  • ab74043 staining TRP2 / DCT in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using a TRIS/EDTA buffer pH 9.0. Samples were then permeabilized, followed by incubation with the primary antibody at a 1/1000 dilution for 30 minutes at 20°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

  • ab74073 staining TRP2 / DCT in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-TRP2 / DCT antibody (ab74073) at 1/500 dilution

    Lane 1 : Extracts from K562 cells with no peptide
    Lane 2 : Extracts from K562 cells with peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa
    Additional bands at: 117 kDa, 39 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescence analysis of TRP2 / DCT in A549 cells, using ab74073 at a dilution of 1/500.
    Left hand panel: no peptide
    right hand panel: with immunising peptide.

References

This product has been referenced in:
  • Marciel MP  et al. Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis. Oncotarget 9:13407-13422 (2018). Read more (PubMed: 29568366) »
  • Kang T  et al. Necroptotic cancer cells-mimicry nanovaccine boosts anti-tumor immunity with tailored immune-stimulatory modality. Biomaterials 164:80-97 (2018). Read more (PubMed: 29499438) »
See all 20 Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Ahmar Aziz

Verified customer

Submitted Feb 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (melan-ink melanocytes)
Specification
melan-ink melanocytes
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Mr. Heiko Locher

Verified customer

Submitted Sep 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
Yes - 0.1% TritonX-100
Specification
skin
Blocking step
3% BSA, 5% NGS, 0.2%TritonX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (B16 mouse melanoma cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
B16 mouse melanoma cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Dr. James Harris

Verified customer

Submitted Sep 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9.0
Permeabilization
Yes - Wash Buffer from Dako
Specification
skin
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Apr 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
10000 cells
Treatment
44 hrs post transfection
Specification
HEK293
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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