Product nameAnti-TRP2/DCT antibody
See all TRP2/DCT primary antibodies
DescriptionRabbit polyclonal to TRP2/DCT
ab74073 detects endogenous levels of total TRP2/DCT protein.
Tested applicationsSuitable for: IHC-Fr, IHC-P, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human TRP2/DCT (internal sequence). synthesized peptide derived from internal of human TRP2.
- WB: Extracts from K562 cells ICC/IF: A549 cells
Previously labelled as TRP2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab74073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 59 kDa.
TRP2 protein is a single-pass type I membrane protein, these types of proteins known to aggregate when boiled therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70C instead of boiling.
|ICC/IF||1/100 - 1/500.|
FunctionInvolved in regulating eumelanin and phaeomelanin levels.
PathwayPigment biosynthesis; melanin biosynthesis.
Sequence similaritiesBelongs to the tyrosinase family.
Cellular localizationMelanosome membrane.
- Information by UniProt
- L dopachrome tautomerase antibody
- DCT antibody
- Dopachrome delta isomerase antibody
ab74043 staining TRP2/DCT in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using a TRIS/EDTA buffer pH 9.0. Samples were then permeabilized, followed by incubation with the primary antibody at a 1/1000 dilution for 30 minutes at 20°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
ab74073 staining TRP2/DCT in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
All lanes : Anti-TRP2/DCT antibody (ab74073) at 1/500 dilution
Lane 1 : Extracts from K562 cells with no peptide
Lane 2 : Extracts from K562 cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 117 kDa, 39 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of TRP2/DCT in A549 cells, using ab74073 at a dilution of 1/500.
Left hand panel: no peptide
right hand panel: with immunising peptide.
This product has been referenced in:
- Liu Y et al. Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes. J Dermatol Sci 93:159-167 (2019). Read more (PubMed: 30904353) »
- Chatatikun M et al. Anti melanogenic effect of Croton roxburghii and Croton sublyratus leaves in a-MSH stimulated B16F10?cells. J Tradit Complement Med 9:66-72 (2019). Read more (PubMed: 30671368) »