Recombinant
RabMAb

Recombinant Anti-TRP2/DCT antibody [EPR21986] (ab221144)

Overview

  • Product name

    Anti-TRP2/DCT antibody [EPR21986]
    See all TRP2/DCT primary antibodies
  • Description

    Rabbit monoclonal [EPR21986] to TRP2/DCT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human TRP2/DCT aa 250-450. The exact sequence is proprietary.
    Database link: P40126

  • Positive control

    • WB: MeWo, SK-MEL-2, SK-MEL-28 and F9 whole cell lysates. IHC-P: Human skin and melanoma tissues. IP: SK-MEL-2 and B16-F0 whole cell lysates.
  • General notes

    Previously labelled as TRP2

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 69, 85 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Recommend for human tissues only.

IP 1/30.

Target

  • Function

    Involved in regulating eumelanin and phaeomelanin levels.
  • Pathway

    Pigment biosynthesis; melanin biosynthesis.
  • Sequence similarities

    Belongs to the tyrosinase family.
  • Cellular localization

    Melanosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • L dopachrome tautomerase antibody
    • DCT antibody
    • Dopachrome delta isomerase antibody
    • Dopachrome tautomerase antibody
    • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
    • DT antibody
    • EC 5.3.3.12 antibody
    • L dopachrome Delta isomerase antibody
    • L-dopachrome Delta-isomerase antibody
    • L-dopachrome tautomerase antibody
    • TRP 2 antibody
    • TRP-2 antibody
    • TRP2 antibody
    • Tyrosinase related protein 2 antibody
    • Tyrosinase-related protein 2 antibody
    • TYRP2 antibody
    • TYRP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRP2/DCT antibody [EPR21986] (ab221144) at 1/1000 dilution

    Lane 1 : MeWo (human malignant melanoma cell line) whole cell lysate
    Lane 2 : SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate
    Lane 3 : SK-MEL-28 (human malignant melanoma cell line) whole cell lysate
    Lane 4 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa
    Observed band size: 69,85 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time : Lane 1: 5 seconds; Lane 2: 15 seconds; Lane 3: 26 seconds; Lane 4: 3 minutes.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 12719423, PMID: 25009701)

  • Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human melanoma (PMID: 24475287). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • TRP2/DCT was immunoprecipitated from 0.35 mg B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: B16-F0 whole cell lysate 10 µg (Input).

    Lane 2: ab221144 IP in B16-F0 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221144 in B16-F0 whole cell lysate.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds.

    ~85KD band is the glycosylated form (PMID: 12719423)

  • TRP2/DCT was immunoprecipitated from 0.35 mg SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: SK-MEL-2 whole cell lysate 10 µg (Input).

    Lane 2: ab221144 IP in SK-MEL-2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221144 in SK-MEL-2 whole cell lysate.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in melanocytes of human skin (PMID:12535195). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab221144 has not yet been referenced specifically in any publications.

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