Recombinant Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free (ab234901)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21986] to TRP2/DCT - BSA and Azide free
- Suitable for: mIHC, WB, IHC-P, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TRP2/DCT antibody [EPR21986] - BSA and Azide free
See all TRP2/DCT primary antibodies -
Description
Rabbit monoclonal [EPR21986] to TRP2/DCT - BSA and Azide free -
Host species
Rabbit -
Specificity
Mouse species is recommended based on WB and IP results, we do not guarantee IHC-P for mouse.
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Tested applications
Suitable for: mIHC, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human melanoma tissue. mIHC: Human skin (melanocytes).
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General notes
ab234901 is the carrier-free version of ab221144.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21986 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
mIHC |
Use at an assay dependent concentration.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 69, 85 kDa (predicted molecular weight: 59 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Mouse species is recommended based on WB and IP results, we do not guarantee IHC-P for mouse. |
|
IP |
Use at an assay dependent concentration.
|
Notes |
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mIHC
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 69, 85 kDa (predicted molecular weight: 59 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Mouse species is recommended based on WB and IP results, we do not guarantee IHC-P for mouse. |
IP
Use at an assay dependent concentration. |
Target
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Function
Involved in regulating eumelanin and phaeomelanin levels. -
Pathway
Pigment biosynthesis; melanin biosynthesis. -
Sequence similarities
Belongs to the tyrosinase family. -
Cellular localization
Melanosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1638 Human
- Entrez Gene: 13190 Mouse
- Omim: 191275 Human
- SwissProt: P40126 Human
- SwissProt: P29812 Mouse
- Unigene: 301865 Human
- Unigene: 19987 Mouse
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Alternative names
- L dopachrome tautomerase antibody
- DCT antibody
- Dopachrome delta isomerase antibody
see all
Images
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TRP2/DCT was immunoprecipitated from 0.35 mg B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: B16-F0 whole cell lysate 10 µg (Input).
Lane 2: ab221144 IP in B16-F0 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221144 in B16-F0 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
~85KD band is the glycosylated form (PMID: 12719423)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221144).
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221144).
Panel A: merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin.
Panel B: anti-TRP2/DCT stained on melanocytes.
Panel C: anti-Langerin stained on Langerhans cells.
Panel D: anti-Desmoglein 1/DSG1 stained on epidermis.The section was incubated in three rounds of staining: in the order of ab236259, ab283686, and ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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TRP2/DCT was immunoprecipitated from 0.35 mg SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate with ab221144 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221144 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: SK-MEL-2 whole cell lysate 10 µg (Input).
Lane 2: ab221144 IP in SK-MEL-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221144 in SK-MEL-2 whole cell lysate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221144).
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in melanocytes of human skin (PMID:12535195). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221144).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP2/DCT with ab221144 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human melanoma (PMID: 24475287). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221144).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab234901 has not yet been referenced specifically in any publications.