Overview

  • Product name

  • Description

    Rabbit polyclonal to TRPA1/TSA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to TRPA1/TSA (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab204483)

  • Positive control

    • DRG cells from human spinal cord
  • General notes

     This product was previously labelled as TRPA1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab62053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 - 10 µg/ml.

Target

  • Function

    Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
  • Tissue specificity

    Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family.
    Contains 15 ANK repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANKTM 1 antibody
    • ANKTM1 antibody
    • Ankyrin-like with transmembrane domains protein 1 antibody
    • Transformation sensitive protein p120 antibody
    • Transformation-sensitive protein p120 antibody
    • Transient receptor potential cation channel subfamily A member 1 antibody
    • TRPA 1 antibody
    • Trpa1 antibody
    • TRPA1_HUMAN antibody
    see all

Images

  • ab62053, at 20µg/ml, staining TRPA1/TSA in Formalin/PFA Fixed Paraffin-Embedded Human DRG tissue cells by Immunohistochemistry.

  • ab62053 staining TRPA1/TSA in murine skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized with 0.4% Triton X-100, blocked using 10% serum for 30 minutes at 25°C and then incubated with ab62053 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:

  • Minami A  et al. Hyperbaric oxygen significantly improves frequent urination, hyperalgesia, and tissue damage in a mouse long-lasting cystitis model induced by an intravesical instillation of hydrogen peroxide. Neurourol Urodyn 38:97-106 (2019). Read more (PubMed: 30411813) »
  • Hashimoto T  et al. Pathophysiological mechanisms of itch in bullous pemphigoid. J Am Acad Dermatol N/A:N/A (2019). Read more (PubMed: 31351883) »
See all 6 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (8%)
Loading amount
7 µg
Specification
Brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 23°C

Mr. KWANGHYUN HUR

Verified customer

Submitted Apr 09 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tendon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako High pH
Permeabilization
No
Specification
Tendon
Blocking step
Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 20 2014

Answer

Thank you for your email.

Yes, we do haveone other antibody against TRPA1 which has been tested in frozen sections and on human and mouse tissue - it is the ab62053. https://www.abcam.com/index.html?datasheet=62053 (or use the following: https://www.abcam.com/index.html?datasheet=62053).

Please let me know if this antibody suits you. Otherwise we have also several antibodies which have been tested on frozen sections, but only either on mouse or human, as for example the ab68847. https://www.abcam.com/index.html?datasheet=68847 (or use the following: https://www.abcam.com/index.html?datasheet=68847).

I look forward to hear back from you.

Read More

Question


I have tried the TRPA1 antibody (ab62053) for a few times. It didn't produce any staining on human and mice intestine frozen sections. When I applied it on mouse DRG cell culture, it seemed to stain everything (see attached images). It has been estimated that only a small proportion of DRG expressing TRPA1. Below is my protocol. Do you have any suggestions? Thanks.

General Information
Antibody storage conditions (temperature/reconstitution etc)
-20℃ after aliquot

Description of the problem (high background, low signal, non-specific satining etc.)
could not get any staining on human and mouse frozen sections. I got some blurry staining on mouse DRG cells, but seemed to stain everything

Sample (Species/Tissue/Cell Type/Cell Line etc.)
human/ mouse intestine, frozen sections, 10 microns
moue DRG cells

Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)
4% PFA overnight in fridge for tissue; 10 minutes at RT for DRG cells
Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
no (frozen section)

Permeabilization step
0.1%Triton X for 10 minutes

Blocking conditions (Buffer/time period, Blocking agent etc.)
5% goat serum block 20 minutes

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
rabbit anti-human TRPA1, fromABCam, 1:50, 1:200, 1:500 in 1% BSA,
incubate overnight in fridge, wash in PBS 3x5 min

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Vector, goat anti-rabbit, 1:200 in 1% BSA, incubate 2 hours at room temperature, wash in PBS 3x5 min

Detection method
secondary antibody conjugated with FITC or CY3
Positive and negative controls used (please specify)
positive: blurry staining on mouse DRG
negative: without primary antibody, acceptable background
Optimization attempts (problem solving)
How many times have you tried the IHC?
5 times
Have you run a "No Primary" control?
yes, no problem
Do you obtain the same results every time?
yes,

Read More
Answer

Thank you for contacting us. I am very sorry to hear that also with the ab62053 the results are not satisfying. I have looked at the protocol and the only thing I can suggest, is to try also the Methanol fixation. Indeed, the result on the datasheet has been obtained with Methanol fixation. Methanol and paraformaldehyde fixation are very different and can results in different availability of the epitopes. However as this antibody has also been tested on paraffin embedded, PFA fixed sections, I would expect it also to work on PFA fixed frozen sections- although there was no antigen retrieval.

I am happy to replace this vial again therefore for you. Please let me know how you would like to proceed. I look forward to hear back from you.

Read More

Answer

Thank you very much for your reply. Indeed it is as you say. The ab62053 istested in IHC-Fr and covered thereby by our guarantee. In WB, ab62053 is not tested and not guaranteed. However if you provide the feedback, you will get another antibody. Please see here below for the details of the testing discount.

I will therefore arrange also the replacement of the faulty tube ab58844 with afree tube of ab62053.You will receive shorty a confirmation email in regards to this replacement.



Discount code for testing ab62053 in Western blot:

DISCOUNT CODE: xx
Expiration date:xx

I am very pleased to hear you would like to accept our offer and test ab62053 inWestern blot.This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for [Western blotand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for providing that information.

As ab58844 failed to work on mouse skin sections in IHC-Fr as guaranteed under our Abpromise, I have sent you ab62053 as a replacement, you order number is 1020816. The replacement antibody is also covered under our Abpromise, to work on mouse sections in IHC-Fr.

If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for contacting Abcam.

I am sorry that you are experiencing problems with ab58844.

Would you please be able to provide me with some additional information in case I can make any protocol suggestions. Could you let me know what type of species you are working on, the type of IHC staining being performed, along with antibody concentrations, incubations times and also if any type of antigen retrieval was performed. We do take all complaints about our products seriously and do our best to resolve them to your satisfaction.

The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-Frozen sections, IHC-PFA perfusion fixed, Frozen sectionsand ICC/IF on mouse and rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

I look forward to your response and helping resolve this issue.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin, HR-1)
Permeabilization
Yes - 0.4% TritonX100
Specification
Skin, HR-1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Sep 20 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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