Overview

  • Product name

  • Description

    Goat polyclonal to TRPC1
  • Host species

    Goat
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide corresponding to Human TRPC1 aa 722-733 (internal sequence). (NP_001238774.1).
    Sequence:

    (c)NLKQKRDENYQK


    Database link: P48995

  • Positive control

    • IHC-P: Human prostate and cortex tissues. ICC/IF: HeLa and U-2 OS cells. Flow cyt: Jurkat cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
  • Tissue specificity

    Seems to be ubiquitous.
  • Sequence similarities

    Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
    Contains 4 ANK repeats.
  • Post-translational
    modifications

    Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • HTRP 1 antibody
    • HTRP1 antibody
    • MGC133334 antibody
    • MGC133335 antibody
    • Mtrp1 antibody
    • Short transient receptor potential channel 1 antibody
    • Transient receptor potential canonical 1 antibody
    • Transient receptor potential cation channel subfamily C member 1 antibody
    • Transient receptor potential channel 1 antibody
    • Transient receptor protein 1 antibody
    • TRP 1 antibody
    • TRP 1 protein antibody
    • Trp related protein 1 antibody
    • TRP-1 antibody
    • TRP1 antibody
    • TRP1 protein antibody
    • TrpC1 antibody
    • Trpc1 transient receptor potential cation channel subfamily C member 1 antibody
    • TRPC1_HUMAN antibody
    • Trrp1 antibody
    see all

Images

  • Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TRPC1 with ab238255 at 10 μg/ml in ICC/IF, followed by AlexaFluor® 488 conjugated  secondary antibody at 4 μg/ml (green). The nuclear counter stain is DAPI (blue).

    -ve control: Unimmunized Goat IgG at 10 μg/ml, followed by AlexaFluor® 488 conjugated  secondary antibody at 4 μg/ml.

  • Paraffin-embedded human prostate tissue stained for TRPC1 using ab238255 at 3.75 μg/ml in immunohistochemical analysis. AP staining.

  • Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling TRPC1 with ab238255 at 10 μg/ml in ICC/IF, followed by AlexaFluor® 488 conjugated  secondary antibody at 4 μg/ml (green). The nuclear counter stain is DAPI (blue).

    -ve control: Unimmunized Goat IgG at 10 μg/ml, followed by AlexaFluor® 488 conjugated  secondary antibody at 4 μg/ml.

  • Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling TRPC1 with ab238255 at 10 μg/ml (blue) compared with unimmunized Goat IgG (black). An AlexaFluor®488 secondary antibody was used at 2 μg/ml.

  • Paraffin-embedded human cortex tissue stained for TRPC1 using ab238255 at 3.75 μg/ml in immunohistochemical analysis. AP staining.

References

ab238255 has not yet been referenced specifically in any publications.

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