Product nameAnti-TRPC1 antibody [EP1417Y]
See all TRPC1 primary antibodies
DescriptionRabbit monoclonal [EP1417Y] to TRPC1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to Human TRPC1.
- HepG2 whole cell lysate (ab7900).
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab51255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
Tissue specificitySeems to be ubiquitous.
Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
Contains 4 ANK repeats.
modificationsActivation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.
- Information by UniProt
- HTRP 1 antibody
- HTRP1 antibody
- MGC133334 antibody
Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/10000 dilution + HepG2 cell lysate at 10 µg
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 91 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/500 dilution (for 16 hours) + Rat artery tissue lysate at 50 µg
An HRP-conjugated Goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: ~100 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Blocking Step: 5% Milk for 16 hours
This product has been referenced in:
- Mazzotta C et al. Proangiogenic effects of soluble a-Klotho on systemic sclerosis dermal microvascular endothelial cells. Arthritis Res Ther 19:27 (2017). Read more (PubMed: 28183357) »
- Xu F et al. Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease. Medicine (Baltimore) 96:e8166 (2017). Read more (PubMed: 29068985) »