• Product name

    Anti-TRPC1 antibody [EP1417Y]
    See all TRPC1 primary antibodies
  • Description

    Rabbit monoclonal [EP1417Y] to TRPC1
  • Host species

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPC1.

  • Positive control

    • HepG2 whole cell lysate (ab7900).
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab51255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
IP 1/100.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IHC.
  • Target

    • Function

      Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
    • Tissue specificity

      Seems to be ubiquitous.
    • Sequence similarities

      Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
      Contains 4 ANK repeats.
    • Post-translational

      Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • HTRP 1 antibody
      • HTRP1 antibody
      • MGC133334 antibody
      • MGC133335 antibody
      • Mtrp1 antibody
      • Short transient receptor potential channel 1 antibody
      • Transient receptor potential canonical 1 antibody
      • Transient receptor potential cation channel subfamily C member 1 antibody
      • Transient receptor potential channel 1 antibody
      • Transient receptor protein 1 antibody
      • TRP 1 antibody
      • TRP 1 protein antibody
      • Trp related protein 1 antibody
      • TRP-1 antibody
      • TRP1 antibody
      • TRP1 protein antibody
      • TrpC1 antibody
      • Trpc1 transient receptor potential cation channel subfamily C member 1 antibody
      • TRPC1_HUMAN antibody
      • Trrp1 antibody
      see all


    • Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/10000 dilution + HepG2 cell lysate at 10 µg

      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 91 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?

    • Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/500 dilution (for 16 hours) + Rat artery tissue lysate at 50 µg

      An HRP-conjugated Goat anti-rabbit polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 91 kDa
      Observed band size: ~100 kDa why is the actual band size different from the predicted?

      Exposure time: 5 minutes

      Blocking Step: 5% Milk for 16 hours

      See Abreview


    This product has been referenced in:

    • Mazzotta C  et al. Proangiogenic effects of soluble a-Klotho on systemic sclerosis dermal microvascular endothelial cells. Arthritis Res Ther 19:27 (2017). Read more (PubMed: 28183357) »
    • Xu F  et al. Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease. Medicine (Baltimore) 96:e8166 (2017). Read more (PubMed: 29068985) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    Western blot
    Rat Tissue lysate - other (artery)
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Sep 07 2010

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up