Recombinant Anti-TRPC1 antibody [EPR1417(2)] - BSA and Azide free (ab251109)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1417(2)] to TRPC1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TRPC1 antibody [EPR1417(2)] - BSA and Azide free
See all TRPC1 primary antibodies -
Description
Rabbit monoclonal [EPR1417(2)] to TRPC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251109 is the carrier-free version of ab192031.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1417(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa). |
Target
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Function
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion. -
Tissue specificity
Seems to be ubiquitous. -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
Contains 4 ANK repeats. -
Post-translational
modificationsActivation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7220 Human
- Entrez Gene: 22063 Mouse
- Entrez Gene: 89821 Rat
- Omim: 602343 Human
- SwissProt: P48995 Human
- SwissProt: Q61056 Mouse
- SwissProt: Q9QX01 Rat
- Unigene: 250687 Human
see all -
Alternative names
- HTRP 1 antibody
- HTRP1 antibody
- MGC133334 antibody
see all
Images
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All lanes : Anti-TRPC1 antibody [EPR1417(2)] (ab192031) at 1/10000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : HepG2 lysate
Lane 3 : Caco-2 lysate
Lane 4 : Human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab192031, the same antibody clone in a different buffer formulation.
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All lanes : Anti-TRPC1 antibody [EPR1417(2)] (ab192031) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : PC-12 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab192031, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251109 has not yet been referenced specifically in any publications.