Overview

  • Product name

  • Description

    Rabbit polyclonal to TRPC3
  • Host species

    Rabbit
  • Specificity

    ab51560 can be used for the detection of TRPC3.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to 14 amino acids near to the N terminal of Human TRPC3

  • Positive control

    • Mouse heart tissue and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab51560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).

Target

  • Relevance

    TRPC channels form the subfamily of channels in human most closely related to drosophila TRP channels. Structurally, this family shares a number of similar characteristics. At the proximal C-terminus of this sub-family is a TRP box motif containing the invariant EWKFAR sequence and between 3 and 4 ankyrin repeats near the N-terminus. These channels are non-selectively permeable to cations, with a selectivity of calcium over sodium variable among the different members. Many of TRPC channel subunits are able to coassemble.
  • Cellular localization

    Cell Membrane; multi-pass membrane protein
  • Database links

  • Alternative names

    • Htrp 3 antibody
    • Htrp3 antibody
    • MGC124333 antibody
    • Mtrp3 antibody
    • Receptor activated cation channel TRP3 antibody
    • Short transient receptor potential channel 3 antibody
    • Transient receptor potential cation channel subfamily C member 3 antibody
    • transient receptor potential cation channel subfamily C member 3 variant c antibody
    • Transient receptor potential channel 3 antibody
    • TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody
    • Transient receptor protein 3 antibody
    • Trp related protein 3 antibody
    • TRP3 antibody
    • TrpC3 antibody
    • Trrp3 antibody
    see all

Images

  • Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody ab51560 at 10 µg/ml.

References

This product has been referenced in:

  • Deurloo MHS  et al. Transcription Factor 2I Regulates Neuronal Development via TRPC3 in 7q11.23 Disorder Models. Mol Neurobiol 56:3313-3325 (2019). Read more (PubMed: 30120731) »
  • López E  et al. Stanniocalcin 2 Regulates Non-capacitative Ca2+ Entry and Aggregation in Mouse Platelets. Front Physiol 9:266 (2018). Mouse . Read more (PubMed: 29628897) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

The exact sequence is proprietary information, but we would be happy to run an alignment and forward the results.


Please let us know if you have any more questions.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: dako low pH
Permeabilization
No
Blocking step
No blocking step used for 30 minute(s) · Concentration: 2%

Dr. Mark Southwood

Verified customer

Submitted Oct 27 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (platelets)
Loading amount
20 µg
Specification
platelets
Treatment
Thrombin 0.1U/ml for 3 min
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Dr. Dr Juan A. Rosado

Verified customer

Submitted Apr 07 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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